pAAV-G-PGK-saCas9-hGH-amp
| Vector Name | pAAV-G-PGK-saCas9-hGH-amp |
| VectorType | AAV Vector |
| Antibiotic Information | Ampicillin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgccc tagggcacca tatgcggtgt gaccggtagg cgccaaccgg 181 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 241 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 301 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 361 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg 421 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 481 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 541 cgggcctttc gacgaattcg ccaccatggc cccaaagaag aagcggaagg tcggtatcca 601 cggagtccca gcagccaagc ggaactacat cctgggcctg gacatcggca tcaccagcgt 661 gggctacggc atcatcgact acgagacacg ggacgtgatc gatgccggcg tgcggctgtt 721 caaagaggcc aacgtggaaa acaacgaggg caggcggagc aagagaggcg ccagaaggct 781 gaagcggcgg aggcggcata gaatccagag agtgaagaag ctgctgttcg actacaacct 841 gctgaccgac cacagcgagc tgagcggcat caacccctac gaggccagag tgaagggcct 901 gagccagaag ctgagcgagg aagagttctc tgccgccctg ctgcacctgg ccaagagaag 961 aggcgtgcac aacgtgaacg aggtggaaga ggacaccggc aacgagctgt ccaccaaaga 1021 gcagatcagc cggaacagca aggccctgga agagaaatac gtggccgaac tgcagctgga 1081 acggctgaag aaagacggcg aagtgcgggg cagcatcaac agattcaaga ccagcgacta 1141 cgtgaaagaa gccaaacagc tgctgaaggt gcagaaggcc taccaccagc tggaccagag 1201 cttcatcgac acctacatcg acctgctgga aacccggcgg acctactatg agggacctgg 1261 cgagggcagc cccttcggct ggaaggacat caaagaatgg tacgagatgc tgatgggcca 1321 ctgcacctac ttccccgagg aactgcggag cgtgaagtac gcctacaacg ccgacctgta 1381 caacgccctg aacgacctga acaatctcgt gatcaccagg gacgagaacg agaagctgga 1441 atattacgag aagttccaga tcatcgagaa cgtgttcaag cagaagaaga agcccaccct 1501 gaagcagatc gccaaagaaa tcctcgtgaa cgaagaggat attaagggct acagagtgac 1561 cagcaccggc aagcccgagt tcaccaacct gaaggtgtac cacgacatca aggacattac 1621 cgcccggaaa gagattattg agaacgccga gctgctggat cagattgcca agatcctgac 1681 catctaccag agcagcgagg acatccagga agaactgacc aatctgaact ccgagctgac 1741 ccaggaagag atcgagcaga tctctaatct gaagggctat accggcaccc acaacctgag 1801 cctgaaggcc atcaacctga tcctggacga gctgtggcac accaacgaca accagatcgc 1861 tatcttcaac cggctgaagc tggtgcccaa gaaggtggac ctgtcccagc agaaagagat 1921 ccccaccacc ctggtggacg acttcatcct gagccccgtc gtgaagagaa gcttcatcca 1981 gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc ctgcccaacg acatcattat 2041 cgagctggcc cgcgagaaga actccaagga cgcccagaaa atgatcaacg agatgcagaa 2101 gcggaaccgg cagaccaacg agcggatcga ggaaatcatc cggaccaccg gcaaagagaa 2161 cgccaagtac ctgatcgaga agatcaagct gcacgacatg caggaaggca agtgcctgta 2221 cagcctggaa gccatccctc tggaagatct gctgaacaac cccttcaact atgaggtgga 2281 ccacatcatc cccagaagcg tgtccttcga caacagcttc aacaacaagg tgctcgtgaa 2341 gcaggaagaa aacagcaaga agggcaaccg gaccccattc cagtacctga gcagcagcga 2401 cagcaagatc agctacgaaa ccttcaagaa gcacatcctg aatctggcca agggcaaggg 2461 cagaatcagc aagaccaaga aagagtatct gctggaagaa cgggacatca acaggttctc 2521 cgtgcagaaa gacttcatca accggaacct ggtggatacc agatacgcca ccagaggcct 2581 gatgaacctg ctgcggagct acttcagagt gaacaacctg gacgtgaaag tgaagtccat 2641 caatggcggc ttcaccagct ttctgcggcg gaagtggaag tttaagaaag agcggaacaa 2701 ggggtacaag caccacgccg aggacgccct gatcattgcc aacgccgatt tcatcttcaa 2761 agagtggaag aaactggaca aggccaaaaa agtgatggaa aaccagatgt tcgaggaaaa 2821 gcaggccgag agcatgcccg agatcgaaac cgagcaggag tacaaagaga tcttcatcac 2881 cccccaccag atcaagcaca ttaaggactt caaggactac aagtacagcc accgggtgga 2941 caagaagcct aatagagagc tgattaacga caccctgtac tccacccgga aggacgacaa 3001 gggcaacacc ctgatcgtga acaatctgaa cggcctgtac gacaaggaca atgacaagct 3061 gaaaaagctg atcaacaaga gccccgaaaa gctgctgatg taccaccacg acccccagac 3121 ctaccagaaa ctgaagctga ttatggaaca gtacggcgac gagaagaatc ccctgtacaa 3181 gtactacgag gaaaccggga actacctgac caagtactcc aaaaaggaca acggccccgt 3241 gatcaagaag attaagtatt acggcaacaa actgaacgcc catctggaca tcaccgacga 3301 ctaccccaac agcagaaaca aggtcgtgaa gctgtccctg aagccctaca gattcgacgt 3361 gtacctggac aatggcgtgt acaagttcgt gaccgtgaag aatctggatg tgatcaaaaa 3421 agaaaactac tacgaagtga atagcaagtg ctatgaggaa gctaagaagc tgaagaagat 3481 cagcaaccag gccgagttta tcgcctcctt ctacaacaac gatctgatca agatcaacgg 3541 cgagctgtat agagtgatcg gcgtgaacaa cgacctgctg aaccggatcg aagtgaacat 3601 gatcgacatc acctaccgcg agtacctgga aaacatgaac gacaagaggc cccccaggat 3661 cattaagaca atcgcctcca agacccagag cattaagaag tacagcacag acattctggg 3721 caacctgtat gaagtgaaat ctaagaagca ccctcagatc atcaaaaagg gcaaaaggcc 3781 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctgac tcgagccaat 3841 cttaaggggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 3901 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 3961 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 4021 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 4081 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 4141 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 4201 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 4261 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 4321 cttcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct 4381 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 4441 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg 4501 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt 4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4621 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 4741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc 4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4861 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat 4921 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta 4981 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct 5041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 5101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 5161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagccgggag ctgcatgtgt 5221 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 5281 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 5461 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 5521 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 5581 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 5641 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 5701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 6001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 6061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 6181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 6481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6601 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 6661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 7021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 7081 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
LOCUS dna 7135 bp FEATURES Location/Qualifiers misc_binding 149..154 /dbxref="REBASE:NdeI" ORF 332..3829 /sequence="ORF_2 rf(2)" ORF 497..3283 /sequence="ORF_3 rf(4)" misc_binding 554..559 /dbxref="REBASE:EcoRI" misc_binding 564..569 /dbxref="REBASE:NcoI" misc_binding 698..703 /dbxref="REBASE:ClaI" misc_binding 1969..1974 /dbxref="REBASE:HindIII" misc_binding 3821..3826 /dbxref="REBASE:BamHI" misc_binding 3830..3835 /dbxref="REBASE:XhoI" Terminator 3847..4323 /gene="hGH_PA term" Rep_Origin 4578..4884 /gene="f1 origin" Promoter 5385..5413 /gene="amp prom" Marker 5455..6315 /gene="amp marker" ORF 5455..6315 /sequence="ORF_1 rf(1)" Rep_Origin 6470..7089 /gene="pBR322 origin" BASE COUNT 1899 a 1918 c 1894 g 1424 t 0 others ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct gcggccgccc tagggcacca tatgcggtgt gaccggtagg cgccaaccgg 181 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc 241 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta 301 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg 361 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg 421 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc 481 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc 541 cgggcctttc gacgaattcg ccaccatggc cccaaagaag aagcggaagg tcggtatcca 601 cggagtccca gcagccaagc ggaactacat cctgggcctg gacatcggca tcaccagcgt 661 gggctacggc atcatcgact acgagacacg ggacgtgatc gatgccggcg tgcggctgtt 721 caaagaggcc aacgtggaaa acaacgaggg caggcggagc aagagaggcg ccagaaggct 781 gaagcggcgg aggcggcata gaatccagag agtgaagaag ctgctgttcg actacaacct 841 gctgaccgac cacagcgagc tgagcggcat caacccctac gaggccagag tgaagggcct 901 gagccagaag ctgagcgagg aagagttctc tgccgccctg ctgcacctgg ccaagagaag 961 aggcgtgcac aacgtgaacg aggtggaaga ggacaccggc aacgagctgt ccaccaaaga 1021 gcagatcagc cggaacagca aggccctgga agagaaatac gtggccgaac tgcagctgga 1081 acggctgaag aaagacggcg aagtgcgggg cagcatcaac agattcaaga ccagcgacta 1141 cgtgaaagaa gccaaacagc tgctgaaggt gcagaaggcc taccaccagc tggaccagag 1201 cttcatcgac acctacatcg acctgctgga aacccggcgg acctactatg agggacctgg 1261 cgagggcagc cccttcggct ggaaggacat caaagaatgg tacgagatgc tgatgggcca 1321 ctgcacctac ttccccgagg aactgcggag cgtgaagtac gcctacaacg ccgacctgta 1381 caacgccctg aacgacctga acaatctcgt gatcaccagg gacgagaacg agaagctgga 1441 atattacgag aagttccaga tcatcgagaa cgtgttcaag cagaagaaga agcccaccct 1501 gaagcagatc gccaaagaaa tcctcgtgaa cgaagaggat attaagggct acagagtgac 1561 cagcaccggc aagcccgagt tcaccaacct gaaggtgtac cacgacatca aggacattac 1621 cgcccggaaa gagattattg agaacgccga gctgctggat cagattgcca agatcctgac 1681 catctaccag agcagcgagg acatccagga agaactgacc aatctgaact ccgagctgac 1741 ccaggaagag atcgagcaga tctctaatct gaagggctat accggcaccc acaacctgag 1801 cctgaaggcc atcaacctga tcctggacga gctgtggcac accaacgaca accagatcgc 1861 tatcttcaac cggctgaagc tggtgcccaa gaaggtggac ctgtcccagc agaaagagat 1921 ccccaccacc ctggtggacg acttcatcct gagccccgtc gtgaagagaa gcttcatcca 1981 gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc ctgcccaacg acatcattat 2041 cgagctggcc cgcgagaaga actccaagga cgcccagaaa atgatcaacg agatgcagaa 2101 gcggaaccgg cagaccaacg agcggatcga ggaaatcatc cggaccaccg gcaaagagaa 2161 cgccaagtac ctgatcgaga agatcaagct gcacgacatg caggaaggca agtgcctgta 2221 cagcctggaa gccatccctc tggaagatct gctgaacaac cccttcaact atgaggtgga 2281 ccacatcatc cccagaagcg tgtccttcga caacagcttc aacaacaagg tgctcgtgaa 2341 gcaggaagaa aacagcaaga agggcaaccg gaccccattc cagtacctga gcagcagcga 2401 cagcaagatc agctacgaaa ccttcaagaa gcacatcctg aatctggcca agggcaaggg 2461 cagaatcagc aagaccaaga aagagtatct gctggaagaa cgggacatca acaggttctc 2521 cgtgcagaaa gacttcatca accggaacct ggtggatacc agatacgcca ccagaggcct 2581 gatgaacctg ctgcggagct acttcagagt gaacaacctg gacgtgaaag tgaagtccat 2641 caatggcggc ttcaccagct ttctgcggcg gaagtggaag tttaagaaag agcggaacaa 2701 ggggtacaag caccacgccg aggacgccct gatcattgcc aacgccgatt tcatcttcaa 2761 agagtggaag aaactggaca aggccaaaaa agtgatggaa aaccagatgt tcgaggaaaa 2821 gcaggccgag agcatgcccg agatcgaaac cgagcaggag tacaaagaga tcttcatcac 2881 cccccaccag atcaagcaca ttaaggactt caaggactac aagtacagcc accgggtgga 2941 caagaagcct aatagagagc tgattaacga caccctgtac tccacccgga aggacgacaa 3001 gggcaacacc ctgatcgtga acaatctgaa cggcctgtac gacaaggaca atgacaagct 3061 gaaaaagctg atcaacaaga gccccgaaaa gctgctgatg taccaccacg acccccagac 3121 ctaccagaaa ctgaagctga ttatggaaca gtacggcgac gagaagaatc ccctgtacaa 3181 gtactacgag gaaaccggga actacctgac caagtactcc aaaaaggaca acggccccgt 3241 gatcaagaag attaagtatt acggcaacaa actgaacgcc catctggaca tcaccgacga 3301 ctaccccaac agcagaaaca aggtcgtgaa gctgtccctg aagccctaca gattcgacgt 3361 gtacctggac aatggcgtgt acaagttcgt gaccgtgaag aatctggatg tgatcaaaaa 3421 agaaaactac tacgaagtga atagcaagtg ctatgaggaa gctaagaagc tgaagaagat 3481 cagcaaccag gccgagttta tcgcctcctt ctacaacaac gatctgatca agatcaacgg 3541 cgagctgtat agagtgatcg gcgtgaacaa cgacctgctg aaccggatcg aagtgaacat 3601 gatcgacatc acctaccgcg agtacctgga aaacatgaac gacaagaggc cccccaggat 3661 cattaagaca atcgcctcca agacccagag cattaagaag tacagcacag acattctggg 3721 caacctgtat gaagtgaaat ctaagaagca ccctcagatc atcaaaaagg gcaaaaggcc 3781 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctgac tcgagccaat 3841 cttaaggggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc 3901 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag 3961 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg 4021 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa 4081 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc 4141 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag 4201 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac 4261 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc 4321 cttcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct 4381 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 4441 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg 4501 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt 4561 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 4621 ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 4681 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 4741 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc 4801 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 4861 gactcttgtt ccaaactgga acaacactca actctatctc gggctattct tttgatttat 4921 aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta 4981 acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct 5041 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 5101 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 5161 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagccgggag ctgcatgtgt 5221 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 5281 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 5461 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 5521 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 5581 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 5641 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 5701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 5761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 5821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 5881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 5941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 6001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 6061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 6121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 6181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 6241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 6301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 6361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 6421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 6481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 6541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 6601 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 6661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 6721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 6781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 6841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 6901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 6961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 7021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 7081 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt //
LOCUS dna 7135 bp FEATURES Location/Qualifiers misc_binding 149..154 /dbxref="REBASE:NdeI" ORF 332..3829 /sequence="ORF_2 rf(2)" ORF 497..3283 /sequence="ORF_3 rf(4)" misc_binding 554..559 /dbxref="REBASE:EcoRI" misc_binding 564..569 /dbxref="REBASE:NcoI" misc_binding 698..703 /dbxref="REBASE:ClaI" misc_binding 1969..1974 /dbxref="REBASE:HindIII" misc_binding 3821..3826 /dbxref="REBASE:BamHI" misc_binding 3830..3835 /dbxref="REBASE:XhoI" Terminator 3847..4323 /gene="hGH_PA term" Rep_Origin 4578..4884 /gene="f1 origin" Promoter 5385..5413 /gene="amp prom" Marker 5455..6315 /gene="amp marker" ORF 5455..6315 /sequence="ORF_1 rf(1)" Rep_Origin 6470..7089 /gene="pBR322 origin" BASE COUNT 1899 a 1918 c 1894 g 1424 t 0 others