pAAV-G-CMV-DIO-SV40-Puro
| Vector Name | pAAV-G-CMV-DIO-SV40-Puro |
| VectorType | AAV Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata 1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac 1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg 1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc 1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct 1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata 1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta 1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg 1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg 1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg 1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc 2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc 2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc 2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag 2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt 2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt 3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc 3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc 3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc 3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct tggtgtggaa 3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccatgacc 3661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc 3721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac 3781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 3841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 3901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 3961 ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa ggagcccgcg 4021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 4141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 4201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactgtgc 4261 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 4321 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 4381 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 4441 agaatagcag gcatgctggg gaaagcttag gaacccctag tgatggagtt ggccactccc 4501 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 4561 tttgcccggg cggcctcagt gagcgagcga gcgcgcaggg atcccggtgt gaaataccgc 4621 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 5881 ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt tcacgtagaa 5941 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac 6001 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata 6061 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc 6121 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg 6181 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga 6241 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 6301 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 6361 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 6421 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 6481 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 6541 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 6601 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 6661 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 6721 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 6781 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 6841 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 6901 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 6961 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 7021 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 7081 tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt gttgttccag 7141 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 7201 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttgcggtcga 7261 ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 7321 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 7381 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc 7441 gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta acatcatcaa 7501 taatatacct t
LOCUS dna 7511 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" Other Gene 1343..1462 /gene="attR1 other" Other Gene 1362..1462 /gene="attR2 other" misc_binding 1469..1474 /dbxref="REBASE:PstI" Other Gene 1503..1808 /gene="ccdB other" Marker 2150..2809 /gene="CAT marker" ORF 2150..2809 /sequence="ORF_3 rf(5)" misc_binding 2591..2596 /dbxref="REBASE:EcoRI" misc_binding 2910..2917 /dbxref="REBASE:NotI" Other Gene 2918..3042 /gene="attR1 other" Other Gene 2918..3018 /gene="attR2 other" Other Gene 3166..3199 /gene="loxP other" misc_binding 3218..3223 /dbxref="REBASE:XbaI" Promoter 3352..3620 /gene="SV40 prom" Rep_Origin 3519..3596 /gene="SV40 origin" ORF 3586..4254 /sequence="ORF_1 rf(1)" misc_binding 3599..3611 /dbxref="REBASE:SfiI" Marker 3655..4254 /gene="puro marker" Rep_Origin 4823..5442 /gene="pBR322 origin" Promoter 6095..6144 /gene="NEOKAN prom" misc_binding 6202..6207 /dbxref="REBASE:BclI" ORF 6233..7027 /sequence="ORF_2 rf(2)" Marker 6236..7024 /gene="NTP_II marker" Rep_Origin 7132..7437 /gene="f1 origin" BASE COUNT 1839 a 1949 c 1930 g 1793 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata 1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac 1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg 1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc 1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct 1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata 1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta 1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg 1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg 1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg 1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc 2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc 2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc 2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag 2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt 2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt 3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc 3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc 3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc 3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct tggtgtggaa 3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccatgacc 3661 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccctcgggc cgtacgcacc 3721 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtggaccc ggaccgccac 3781 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 3841 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 3901 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 3961 ctggccgcgc agcaacagat ggaagggctc ctggcgccgc accggcccaa ggagcccgcg 4021 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 4081 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 4141 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 4201 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgactgtgc 4261 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 4321 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 4381 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 4441 agaatagcag gcatgctggg gaaagcttag gaacccctag tgatggagtt ggccactccc 4501 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 4561 tttgcccggg cggcctcagt gagcgagcga gcgcgcaggg atcccggtgt gaaataccgc 4621 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 4681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 4741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 4801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 4861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 4921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 4981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 5041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 5101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 5161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 5221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 5281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 5341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 5401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 5461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 5521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 5581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 5641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 5701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 5761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 5821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 5881 ttaatagttt gcgcaacgtt gttgaaaaag gatcttcacc tagatccttt tcacgtagaa 5941 agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac 6001 aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata 6061 gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc 6121 tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc caaggatctg 6181 atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc gcatgattga 6241 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 6301 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 6361 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga 6421 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 6481 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 6541 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 6601 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 6661 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 6721 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga 6781 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 6841 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 6901 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 6961 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 7021 cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa 7081 tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt gttgttccag 7141 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg 7201 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttgcggtcga 7261 ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga gcttgacggg 7321 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg 7381 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc ttaatgcgcc 7441 gctacagggc gcgtccattc gccattcagg atcgaattaa ttcttaatta acatcatcaa 7501 taatatacct t //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG 481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540 TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC 541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600 TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT 601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660 TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC 661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720 GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA 721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780 CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT NdeI | 781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840 TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG 841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900 TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT 901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960 GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG 961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC 1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT KpnI | 1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200 GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC 1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260 ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG loxP other(1288,1321)<<< | 1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320 GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT attR2 other(1362,1462)<<< | XhoI attR1 other(1343,1462)>>> | | | 1321 tctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacgtaaaatgata 1380 AGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGCATTTTACTAT 1381 taaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaa 1440 ATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTT PstI | 1441 cacaacatatccagtcactatggtcgacctgcagactggctgtgtataagggagcctgac 1500 GTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTCCCTCGGACTG ccdB other(1503,1808)<<< | 1501 atttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctg 1560 TAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAGCGCCACCGAC 1561 agatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatc 1620 TCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAGCCACCAGTAG 1621 atgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatct 1680 TACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCTCTGAAATAGA 1681 gacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataata 1740 CTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCACAGTTATTAT 1741 tcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaacctta 1800 AGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCACATTTGGAAT 1801 aactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccg 1860 TTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAAGTTATTTGGC 1861 ggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacg 1920 CCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTGCGTCTGCTGC 1921 ggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttg 1980 CCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTATATACGGAAC 1981 agcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctc 2040 TCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTATCGTATGGAG 2041 tttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgc 2100 AAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTTTTTAGTCGCG ORF_3 rf(5)(2150,2809)<<< | CAT marker(2150,2809)<<< | 2101 gcaaatacgcatactgttatctggcttttagtaagccggatccagatctttacgccccgc 2160 CGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAAATGCGGGGCG 2161 cctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccat 2220 GGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGTACCTTCGGTA 2221 cacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtat 2280 GTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATA 2281 aatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaat 2340 TTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTA 2341 caaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaacc 2400 GTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAGAGTTATTTGG 2401 ctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgta 2460 GAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACAT 2461 gaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgct 2520 CTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGA 2521 catggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttca 2580 GTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGT EcoRI | 2581 ttgccatacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccg 2640 AACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGC 2641 gataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaa 2700 CTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTT 2701 cggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgat 2760 GCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTA 2761 gccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttcct 2820 CGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGA 2821 tagctcctgaaaatctcgacggatcctaactcaaaatccacacattatacgagccggaag 2880 ATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATGCTCGGCCTTC attR1 other(2918,3042)<<< | NotI attR2 other(2918,3018)>>> | | 2881 cataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactggatatgttgtgt 2940 GTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCTATACAACACA 2941 tttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttat 3000 AAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATA 3001 atcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcgctagccaatt 3060 TAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGCGATCGGTTAA 3061 gataacttcgtataggatactttatacgaagttatcattgggattcttcctattttgatc 3120 CTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAG loxP other(3166,3199)>>> | 3121 caagcatcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagc 3180 GTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCG XbaI | 3181 atacattatacgaagttatatggtgagcaagggcgattctagaaacttgtttattgcagc 3240 TATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCG 3241 ttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttc 3300 AATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAG SV40 prom(3352,3620)>>> | 3301 actgcattctagttgtggtttgtccaaactcatcaatgtatcttaaagcttggtgtggaa 3360 TGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAACCACACCTT 3361 agtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaa 3420 TCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTT 3421 ccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca 3480 GGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGT SV40 origin(3519,3596)>>> | 3481 attagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgccca 3540 TAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGT ORF_1 rf(1)(3586,4254)>>> | 3541 gttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgagg 3600 CAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCC SfiI puro marker(3655,4254)>>> | | 3601 ccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccatgacc 3660 GGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGG 3661 gagtacaagcccacggtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcacc 3720 CTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGG 3721 ctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtggacccggaccgccac 3780 GAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTG 3781 atcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggc 3840 TAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCG 3841 aaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtc 3900 TTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAG 3901 gaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccgg 3960 CTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCC 3961 ctggccgcgcagcaacagatggaagggctcctggcgccgcaccggcccaaggagcccgcg 4020 GACCGGCGCGTCGTTGTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGC 4021 tggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgcc 4080 ACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGG 4081 gtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacc 4140 CAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGG 4141 tccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtc 4200 AGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAG 4201 gaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgactgtgc 4260 CTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTGACACG 4261 cttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaag 4320 GAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTC 4321 gtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagta 4380 CACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCAT 4381 ggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaag 4440 CCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTC 4441 agaatagcaggcatgctggggaaagcttaggaacccctagtgatggagttggccactccc 4500 TCTTATCGTCCGTACGACCCCTTTCGAATCCTTGGGGATCACTACCTCAACCGGTGAGGG 4501 tctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggc 4560 AGAGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCG 4561 tttgcccgggcggcctcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgc 4620 AAACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCG 4621 acagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgact 4680 TGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGA 4681 cgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatac 4740 GCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATG 4741 ggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaa 4800 CCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTT pBR322 origin(4823,5442)<<< | 4801 aggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctg 4860 TCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGAC 4861 acgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaa 4920 TGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTT 4921 gataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgc 4980 CTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCG 4981 ttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcac 5040 AATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTG 5041 gctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaac 5100 CGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTG 5101 cccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccgg 5160 GGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCC 5161 taagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggt 5220 ATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCA 5221 atgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagga 5280 TACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCT 5281 cagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagct 5340 GTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGA 5341 cttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcaga 5400 GAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCT 5401 ttacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacg 5460 AATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGC 5461 ctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatct 5520 GAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGA 5521 tcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagt 5580 AGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCA 5581 aaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtc 5640 TTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAG 5641 tatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg 5700 ATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCC 5701 gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccag 5760 CGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTC 5761 atttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactt 5820 TAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAA 5821 tatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccag 5880 ATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTC 5881 ttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaa 5940 AATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTT 5941 agccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggac 6000 TCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTG 6001 aagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgata 6060 TTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTAT NEOKAN prom(6095,6144)>>> | 6061 gctagactgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctc 6120 CGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAG 6121 tggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctg 6180 ACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGAC NTP_II marker(6236,7024)>>> | BclI ORF_2 rf(2)(6233,7027)>>> | | | 6181 atggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattga 6240 TACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACT 6241 acaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatga 6300 TGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACT 6301 ctgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggg 6360 GACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCC 6361 gcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacga 6420 CGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCT 6421 ggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgt 6480 CCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCA 6481 tgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcct 6540 ACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGA 6541 gtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggct 6600 CAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGA 6601 gcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcg 6660 CGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGC 6661 agcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatca 6720 TCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGT 6721 ggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgagga 6780 CCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCT 6781 tctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgctt 6840 AGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAA 6841 ttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgtt 6900 AAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAA 6901 ggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgct 6960 CCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGA 6961 ttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagtt 7020 AATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAA 7021 cttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaa 7080 GAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTT f1 origin(7132,7437)<<< | 7081 tcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccag 7140 AGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTC 7141 tttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccg 7200 AAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGC 7201 tctatcagggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcga 7260 AGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCT 7261 ggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggg 7320 CCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCC 7321 gaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctaggg 7380 CTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCC 7381 cgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgcc 7440 GCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGG 7441 gctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaa 7500 CGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTT 7501 taatatacctt 7511 ATTATATGGAA
LOCUS dna 7511 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" Other Gene 1343..1462 /gene="attR1 other" Other Gene 1362..1462 /gene="attR2 other" misc_binding 1469..1474 /dbxref="REBASE:PstI" Other Gene 1503..1808 /gene="ccdB other" Marker 2150..2809 /gene="CAT marker" ORF 2150..2809 /sequence="ORF_3 rf(5)" misc_binding 2591..2596 /dbxref="REBASE:EcoRI" misc_binding 2910..2917 /dbxref="REBASE:NotI" Other Gene 2918..3042 /gene="attR1 other" Other Gene 2918..3018 /gene="attR2 other" Other Gene 3166..3199 /gene="loxP other" misc_binding 3218..3223 /dbxref="REBASE:XbaI" Promoter 3352..3620 /gene="SV40 prom" Rep_Origin 3519..3596 /gene="SV40 origin" ORF 3586..4254 /sequence="ORF_1 rf(1)" misc_binding 3599..3611 /dbxref="REBASE:SfiI" Marker 3655..4254 /gene="puro marker" Rep_Origin 4823..5442 /gene="pBR322 origin" Promoter 6095..6144 /gene="NEOKAN prom" misc_binding 6202..6207 /dbxref="REBASE:BclI" ORF 6233..7027 /sequence="ORF_2 rf(2)" Marker 6236..7024 /gene="NTP_II marker" Rep_Origin 7132..7437 /gene="f1 origin" BASE COUNT 1839 a 1949 c 1930 g 1793 t 0 others