pAAV-G-CMV-DIO-SV40-GFP
| Vector Name | pAAV-G-CMV-DIO-SV40-GFP |
| VectorType | AAV Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata 1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac 1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg 1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc 1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct 1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata 1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta 1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg 1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg 1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg 1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc 2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc 2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc 2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag 2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt 2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt 3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc 3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc 3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc 3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct tggtgtggaa 3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccgccacc 3661 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3721 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 3781 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3841 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3901 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3961 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4021 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4081 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4141 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4201 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4261 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4321 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4381 gacggcaccg ccggacccgg ctccaccgga tctcgctgac tgtgccttct agttgccagc 4441 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 4501 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 4561 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg 4621 ctggggaaag cttaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 4681 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 4741 tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga tgcgtaagga 4801 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5401 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 5461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 6001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 6061 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa 6121 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag 6181 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt 6241 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa 6301 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc 6361 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 6421 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 6481 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 6541 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 6601 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 6661 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 6721 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 6781 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 6841 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 6901 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 6961 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 7021 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 7081 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 7141 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt 7201 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt 7261 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc 7321 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 7381 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc 7441 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 7501 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 7561 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc 7621 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt
LOCUS dna 7676 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" Other Gene 1343..1462 /gene="attR1 other" Other Gene 1362..1462 /gene="attR2 other" Other Gene 1503..1808 /gene="ccdB other" Marker 2150..2809 /gene="CAT marker" ORF 2150..2809 /sequence="ORF_3 rf(5)" misc_binding 2591..2596 /dbxref="REBASE:EcoRI" misc_binding 2910..2917 /dbxref="REBASE:NotI" Other Gene 2918..3042 /gene="attR1 other" Other Gene 2918..3018 /gene="attR2 other" Other Gene 3166..3199 /gene="loxP other" misc_binding 3218..3223 /dbxref="REBASE:XbaI" Promoter 3352..3620 /gene="SV40 prom" Rep_Origin 3519..3596 /gene="SV40 origin" ORF 3586..4419 /sequence="ORF_1 rf(1)" misc_binding 3599..3611 /dbxref="REBASE:SfiI" misc_binding 4245..4250 /dbxref="REBASE:ApaI" Rep_Origin 4988..5607 /gene="pBR322 origin" Promoter 6260..6309 /gene="NEOKAN prom" misc_binding 6367..6372 /dbxref="REBASE:BclI" ORF 6398..7192 /sequence="ORF_2 rf(2)" Marker 6401..7189 /gene="NTP_II marker" Rep_Origin 7297..7602 /gene="f1 origin" BASE COUNT 1908 a 1992 c 1948 g 1828 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata 1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac 1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg 1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc 1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct 1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata 1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta 1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg 1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg 1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg 1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc 2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc 2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc 2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag 2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt 2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt 3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc 3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc 3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc 3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct tggtgtggaa 3361 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 3421 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 3481 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 3541 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 3601 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggccgccacc 3661 atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat caccggcacc 3721 ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa gcagggccgc 3781 atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta cctgctgagc 3841 cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta cgagaacccc 3901 ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa gtacgaggac 3961 ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt gatcggcgac 4021 ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga caagatcatc 4081 cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct ggtgggcagc 4141 ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt ggacagccac 4201 atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc catgttcgcc 4261 ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga gtaccagcac 4321 gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa ttctgccgtg 4381 gacggcaccg ccggacccgg ctccaccgga tctcgctgac tgtgccttct agttgccagc 4441 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 4501 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 4561 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg 4621 ctggggaaag cttaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 4681 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 4741 tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga tgcgtaagga 4801 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 5401 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 5461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 6001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 6061 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa 6121 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag 6181 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt 6241 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa 6301 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc 6361 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 6421 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 6481 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 6541 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 6601 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 6661 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 6721 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 6781 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 6841 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 6901 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 6961 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 7021 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 7081 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 7141 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt 7201 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt 7261 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc 7321 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 7381 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc 7441 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 7501 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 7561 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc 7621 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG 481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540 TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC 541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600 TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT 601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660 TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC 661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720 GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA 721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780 CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT NdeI | 781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840 TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG 841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900 TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT 901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960 GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG 961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC 1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT KpnI | 1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200 GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC 1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260 ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG loxP other(1288,1321)<<< | 1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320 GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT attR2 other(1362,1462)<<< | XhoI attR1 other(1343,1462)>>> | | | 1321 tctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacgtaaaatgata 1380 AGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGCATTTTACTAT 1381 taaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaa 1440 ATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTT 1441 cacaacatatccagtcactatggtcgacctgcagactggctgtgtataagggagcctgac 1500 GTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTCCCTCGGACTG ccdB other(1503,1808)<<< | 1501 atttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctg 1560 TAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAGCGCCACCGAC 1561 agatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatc 1620 TCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAGCCACCAGTAG 1621 atgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatct 1680 TACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCTCTGAAATAGA 1681 gacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataata 1740 CTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCACAGTTATTAT 1741 tcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaacctta 1800 AGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCACATTTGGAAT 1801 aactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccg 1860 TTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAAGTTATTTGGC 1861 ggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacg 1920 CCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTGCGTCTGCTGC 1921 ggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttg 1980 CCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTATATACGGAAC 1981 agcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctc 2040 TCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTATCGTATGGAG 2041 tttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgc 2100 AAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTTTTTAGTCGCG ORF_3 rf(5)(2150,2809)<<< | CAT marker(2150,2809)<<< | 2101 gcaaatacgcatactgttatctggcttttagtaagccggatccagatctttacgccccgc 2160 CGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAAATGCGGGGCG 2161 cctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccat 2220 GGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGTACCTTCGGTA 2221 cacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtat 2280 GTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATA 2281 aatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaat 2340 TTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTA 2341 caaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaacc 2400 GTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAGAGTTATTTGG 2401 ctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgta 2460 GAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACAT 2461 gaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgct 2520 CTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGA 2521 catggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttca 2580 GTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGT EcoRI | 2581 ttgccatacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccg 2640 AACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGC 2641 gataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaa 2700 CTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTT 2701 cggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgat 2760 GCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTA 2761 gccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttcct 2820 CGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGA 2821 tagctcctgaaaatctcgacggatcctaactcaaaatccacacattatacgagccggaag 2880 ATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATGCTCGGCCTTC attR2 other(2918,3018)>>> | NotI attR1 other(2918,3042)<<< | | 2881 cataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactggatatgttgtgt 2940 GTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCTATACAACACA 2941 tttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttat 3000 AAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATA 3001 atcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcgctagccaatt 3060 TAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGCGATCGGTTAA 3061 gataacttcgtataggatactttatacgaagttatcattgggattcttcctattttgatc 3120 CTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAG loxP other(3166,3199)>>> | 3121 caagcatcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagc 3180 GTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCG XbaI | 3181 atacattatacgaagttatatggtgagcaagggcgattctagaaacttgtttattgcagc 3240 TATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCG 3241 ttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttc 3300 AATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAG SV40 prom(3352,3620)>>> | 3301 actgcattctagttgtggtttgtccaaactcatcaatgtatcttaaagcttggtgtggaa 3360 TGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAACCACACCTT 3361 agtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaa 3420 TCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTT 3421 ccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca 3480 GGTCCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGT SV40 origin(3519,3596)>>> | 3481 attagtcagcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgccca 3540 TAATCAGTCGTTGGTATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGT ORF_1 rf(1)(3586,4419)>>> | 3541 gttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgagg 3600 CAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCC SfiI | 3601 ccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggccgccacc 3660 GGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGCGGTGG 3661 Atggagagcgacgagagcggcctgcccgccatggagatcgagtgccgcatcaccggcacc 3720 TACCTCTCGCTGCTCTCGCCGGACGGGCGGTACCTCTAGCTCACGGCGTAGTGGCCGTGG 3721 ctgaacggcgtggagttcgagctggtgggcggcggagagggcacccccaagcagggccgc 3780 GACTTGCCGCACCTCAAGCTCGACCACCCGCCGCCTCTCCCGTGGGGGTTCGTCCCGGCG 3781 atgaccaacaagatgaagagcaccaaaggcgccctgaccttcagcccctacctgctgagc 3840 TACTGGTTGTTCTACTTCTCGTGGTTTCCGCGGGACTGGAAGTCGGGGATGGACGACTCG 3841 cacgtgatgggctacggcttctaccacttcggcacctaccccagcggctacgagaacccc 3900 GTGCACTACCCGATGCCGAAGATGGTGAAGCCGTGGATGGGGTCGCCGATGCTCTTGGGG 3901 ttcctgcacgccatcaacaacggcggctacaccaacacccgcatcgagaagtacgaggac 3960 AAGGACGTGCGGTAGTTGTTGCCGCCGATGTGGTTGTGGGCGTAGCTCTTCATGCTCCTG 3961 ggcggcgtgctgcacgtgagcttcagctaccgctacgaggccggccgcgtgatcggcgac 4020 CCGCCGCACGACGTGCACTCGAAGTCGATGGCGATGCTCCGGCCGGCGCACTAGCCGCTG 4021 ttcaaggtggtgggcaccggcttccccgaggacagcgtgatcttcaccgacaagatcatc 4080 AAGTTCCACCACCCGTGGCCGAAGGGGCTCCTGTCGCACTAGAAGTGGCTGTTCTAGTAG 4081 cgcagcaacgccaccgtggagcacctgcaccccatgggcgataacgtgctggtgggcagc 4140 GCGTCGTTGCGGTGGCACCTCGTGGACGTGGGGTACCCGCTATTGCACGACCACCCGTCG 4141 ttcgcccgcaccttcagcctgcgcgacggcggctactacagcttcgtggtggacagccac 4200 AAGCGGGCGTGGAAGTCGGACGCGCTGCCGCCGATGATGTCGAAGCACCACCTGTCGGTG ApaI | 4201 atgcacttcaagagcgccatccaccccagcatcctgcagaacgggggccccatgttcgcc 4260 TACGTGAAGTTCTCGCGGTAGGTGGGGTCGTAGGACGTCTTGCCCCCGGGGTACAAGCGG 4261 ttccgccgcgtggaggagctgcacagcaacaccgagctgggcatcgtggagtaccagcac 4320 AAGGCGGCGCACCTCCTCGACGTGTCGTTGTGGCTCGACCCGTAGCACCTCATGGTCGTG 4321 gccttcaagacccccatcgccttcgccagatcccgcgctcagtcgtccaattctgccgtg 4380 CGGAAGTTCTGGGGGTAGCGGAAGCGGTCTAGGGCGCGAGTCAGCAGGTTAAGACGGCAC 4381 gacggcaccgccggacccggctccaccggatctcgctgactgtgccttctagttgccagc 4440 CTGCCGTGGCGGCCTGGGCCGAGGTGGCCTAGAGCGACTGACACGGAAGATCAACGGTCG 4441 catctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactg 4500 GTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGAC 4501 tcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattc 4560 AGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAG 4561 tggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatg 4620 ACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTCTTATCGTCCGTAC 4621 ctggggaaagcttaggaacccctagtgatggagttggccactccctctctgcgcgctcgc 4680 GACCCCTTTCGAATCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCG 4681 tcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcc 4740 AGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGG 4741 tcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgcacagatgcgtaagga 4800 AGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCT 4801 gaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcg 4860 CTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGC 4861 ttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaat 4920 AAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTA 4921 caggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgta 4980 GTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCAT pBR322 origin(4988,5607)<<< | 4981 aaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaa 5040 TTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTT 5041 atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttc 5100 TAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAG 5101 cccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgt 5160 GGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACA 5161 ccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctca 5220 GGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGT 5221 gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg 5280 CAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGC 5281 accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttat 5340 TGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATA 5341 cgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgcta 5400 GCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGAT 5401 cagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatct 5460 GTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGA 5461 gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaac 5520 CGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTG 5521 aaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaa 5580 TTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTT 5581 aaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaa 5640 TTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTT 5641 actcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttt 5700 TGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAA 5701 taaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgaca 5760 ATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGT 5761 gttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcca 5820 CAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGT 5821 tagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcc 5880 ATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGG 5881 ccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataa 5940 GGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATT 5941 accagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcc 6000 TGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGG 6001 agtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgca 6060 TCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGT 6061 acgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaa 6120 TGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTT 6121 acggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaag 6180 TGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTC 6181 cgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggt 6240 GCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCA NEOKAN prom(6260,6309)>>> | 6241 tttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaa 6300 AAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTT 6301 gccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatc 6360 CGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAG NTP_II marker(6401,7189)>>> | BclI ORF_2 rf(2)(6398,7192)>>> | | | 6361 aagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgca 6420 TTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGT 6421 cgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagac 6480 GCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTG 6481 aatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttt 6540 TTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAA 6541 tgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatc 6600 ACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAG 6601 gtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggg 6660 CACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCC 6661 aagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgc 6720 TTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACG 6721 tcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatcc 6780 AGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGG 6781 ggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggat 6840 CCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTA 6841 ggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagc 6900 CCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCG 6901 cgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgaccca 6960 GCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGT 6961 tggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcga 7020 ACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCT 7021 ctgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatat 7080 GACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATA 7081 tgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgc 7140 ACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCG 7141 tcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgtt 7200 AGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAA 7201 aaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatccctt 7260 TTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAA f1 origin(7297,7602)<<< | 7261 ataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtc 7320 TATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAG 7321 cactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatg 7380 GTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTAC 7381 gcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctc 7440 CGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAG 7441 taaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacg 7500 ATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGC 7501 tggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtag 7560 ACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATC 7561 cggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtc 7620 GCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAG 7621 cattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 7676 GTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 7676 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" Other Gene 1343..1462 /gene="attR1 other" Other Gene 1362..1462 /gene="attR2 other" Other Gene 1503..1808 /gene="ccdB other" Marker 2150..2809 /gene="CAT marker" ORF 2150..2809 /sequence="ORF_3 rf(5)" misc_binding 2591..2596 /dbxref="REBASE:EcoRI" misc_binding 2910..2917 /dbxref="REBASE:NotI" Other Gene 2918..3042 /gene="attR1 other" Other Gene 2918..3018 /gene="attR2 other" Other Gene 3166..3199 /gene="loxP other" misc_binding 3218..3223 /dbxref="REBASE:XbaI" Promoter 3352..3620 /gene="SV40 prom" Rep_Origin 3519..3596 /gene="SV40 origin" ORF 3586..4419 /sequence="ORF_1 rf(1)" misc_binding 3599..3611 /dbxref="REBASE:SfiI" misc_binding 4245..4250 /dbxref="REBASE:ApaI" Rep_Origin 4988..5607 /gene="pBR322 origin" Promoter 6260..6309 /gene="NEOKAN prom" misc_binding 6367..6372 /dbxref="REBASE:BclI" ORF 6398..7192 /sequence="ORF_2 rf(2)" Marker 6401..7189 /gene="NTP_II marker" Rep_Origin 7297..7602 /gene="f1 origin" BASE COUNT 1908 a 1992 c 1948 g 1828 t 0 others