pAAV-G-CMV-DIO
| Vector Name | pAAV-G-CMV-DIO |
| VectorType | AAV Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata 1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac 1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg 1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc 1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct 1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata 1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta 1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg 1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg 1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg 1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc 2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc 2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc 2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag 2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt 2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt 3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc 3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc 3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc 3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct taggaacccc 3361 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac 3421 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca 3481 gggatcccgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 3541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 3601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 3661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 3721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 3781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 3841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 3901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 3961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4141 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 4201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 4261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 4381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 4441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 4501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 4561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 4621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 4681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 4741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgaaa aaggatcttc 4801 acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat 4861 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct 4921 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg 4981 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg 5041 gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga 5101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 5161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 5221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 5281 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 5341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 5401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 5461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 5521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 5581 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 5641 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 5701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 5761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 5821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 5881 ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt aaatcagctc 5941 attttttaac caataggccg aaatcggcaa catcccttat aaatcaaaag aatagaccgc 6001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 6061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 6121 caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc ctaaagggag 6181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 6241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 6301 cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat 6361 taattcttaa ttaacatcat caataatata cctt
LOCUS dna 6394 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" Other Gene 1343..1462 /gene="attR1 other" Other Gene 1362..1462 /gene="attR2 other" misc_binding 1469..1474 /dbxref="REBASE:PstI" Other Gene 1503..1808 /gene="ccdB other" Marker 2150..2809 /gene="CAT marker" ORF 2150..2809 /sequence="ORF_2 rf(4)" misc_binding 2591..2596 /dbxref="REBASE:EcoRI" misc_binding 2910..2917 /dbxref="REBASE:NotI" Other Gene 2918..3042 /gene="attR1 other" Other Gene 2918..3018 /gene="attR2 other" Other Gene 3166..3199 /gene="loxP other" misc_binding 3218..3223 /dbxref="REBASE:XbaI" misc_binding 3346..3351 /dbxref="REBASE:HindIII" Rep_Origin 3706..4325 /gene="pBR322 origin" Promoter 4978..5027 /gene="NEOKAN prom" misc_binding 5085..5090 /dbxref="REBASE:BclI" ORF 5116..5910 /sequence="ORF_1 rf(1)" Marker 5119..5907 /gene="NTP_II marker" misc_binding 5645..5650 /dbxref="REBASE:SphI" Rep_Origin 6015..6320 /gene="f1 origin" BASE COUNT 1641 a 1569 c 1587 g 1597 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcaacc actttgtaca agaaagctga acgagaaacg taaaatgata 1381 taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa 1441 cacaacatat ccagtcacta tggtcgacct gcagactggc tgtgtataag ggagcctgac 1501 atttatattc cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg 1561 agatcagcca cttcttcccc gataacggag accggcacac tggccatatc ggtggtcatc 1621 atgcgccagc tttcatcccc gatatgcacc accgggtaaa gttcacggga gactttatct 1681 gacagcagac gtgcactggc cagggggatc accatccgtc gcccgggcgt gtcaataata 1741 tcactctgta catccacaaa cagacgataa cggctctctc ttttataggt gtaaacctta 1801 aactgcattt caccagcccc tgttctcgtc agcaaaagag ccgttcattt caataaaccg 1861 ggcgacctca gccatccctt cctgattttc cgctttccag cgttcggcac gcagacgacg 1921 ggcttcattc tgcatggttg tgcttaccag accggagata ttgacatcat atatgccttg 1981 agcaactgat agctgtcgct gtcaactgtc actgtaatac gctgcttcat agcatacctc 2041 tttttgacat acttcgggta tacatatcag tatatattct tataccgcaa aaatcagcgc 2101 gcaaatacgc atactgttat ctggctttta gtaagccgga tccagatctt tacgccccgc 2161 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat 2221 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat 2281 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat 2341 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc 2401 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta 2461 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct 2521 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca 2581 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg 2641 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa 2701 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat 2761 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct 2821 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag 2881 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt 2941 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat 3001 atcattttac gtttctcgtt cagctttttt gtacaaactt gttgatatcg ctagccaatt 3061 gataacttcg tataggatac tttatacgaa gttatcattg ggattcttcc tattttgatc 3121 caagcatcac catcgaccct ctagtccaga tctcaccatc gacccataac ttcgtatagc 3181 atacattata cgaagttata tggtgagcaa gggcgattct agaaacttgt ttattgcagc 3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaagct taggaacccc 3361 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac 3421 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca 3481 gggatcccgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 3541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 3601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 3661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 3721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 3781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 3841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 3901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 3961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 4021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 4081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 4141 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 4201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 4261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 4321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 4381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 4441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 4501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 4561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 4621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 4681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 4741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgaaa aaggatcttc 4801 acctagatcc ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat 4861 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct 4921 tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg 4981 gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg 5041 gctttctcgc cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga 5101 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 5161 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 5221 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 5281 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 5341 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 5401 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 5461 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 5521 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 5581 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 5641 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 5701 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 5761 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 5821 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 5881 ttctatcgcc ttcttgacga gttcttctga attttgttaa aatttttgtt aaatcagctc 5941 attttttaac caataggccg aaatcggcaa catcccttat aaatcaaaag aatagaccgc 6001 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 6061 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 6121 caaatcaagt tttttgcggt cgaggtgccg taaagctcta aatcggaacc ctaaagggag 6181 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 6241 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 6301 cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggatcgaat 6361 taattcttaa ttaacatcat caataatata cctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG 481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540 TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC 541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600 TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT 601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660 TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC 661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720 GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA 721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780 CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT NdeI | 781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840 TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG 841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900 TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT 901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960 GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG 961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC 1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT KpnI | 1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200 GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC 1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260 ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG loxP other(1288,1321)<<< | 1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320 GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT attR2 other(1362,1462)<<< | XhoI attR1 other(1343,1462)>>> | | | 1321 tctcgagtcagatatcaaccactttgtacaagaaagctgaacgagaaacgtaaaatgata 1380 AGAGCTCAGTCTATAGTTGGTGAAACATGTTCTTTCGACTTGCTCTTTGCATTTTACTAT 1381 taaatatcaatatattaaattagattttgcataaaaaacagactacataatactgtaaaa 1440 ATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTT PstI | 1441 cacaacatatccagtcactatggtcgacctgcagactggctgtgtataagggagcctgac 1500 GTGTTGTATAGGTCAGTGATACCAGCTGGACGTCTGACCGACACATATTCCCTCGGACTG ccdB other(1503,1808)<<< | 1501 atttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctg 1560 TAAATATAAGGGGTCTTGTAGTCCAATTACCGCAAAAACTACAGTAAAAGCGCCACCGAC 1561 agatcagccacttcttccccgataacggagaccggcacactggccatatcggtggtcatc 1620 TCTAGTCGGTGAAGAAGGGGCTATTGCCTCTGGCCGTGTGACCGGTATAGCCACCAGTAG 1621 atgcgccagctttcatccccgatatgcaccaccgggtaaagttcacgggagactttatct 1680 TACGCGGTCGAAAGTAGGGGCTATACGTGGTGGCCCATTTCAAGTGCCCTCTGAAATAGA 1681 gacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtcaataata 1740 CTGTCGTCTGCACGTGACCGGTCCCCCTAGTGGTAGGCAGCGGGCCCGCACAGTTATTAT 1741 tcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaacctta 1800 AGTGAGACATGTAGGTGTTTGTCTGCTATTGCCGAGAGAGAAAATATCCACATTTGGAAT 1801 aactgcatttcaccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccg 1860 TTGACGTAAAGTGGTCGGGGACAAGAGCAGTCGTTTTCTCGGCAAGTAAAGTTATTTGGC 1861 ggcgacctcagccatcccttcctgattttccgctttccagcgttcggcacgcagacgacg 1920 CCGCTGGAGTCGGTAGGGAAGGACTAAAAGGCGAAAGGTCGCAAGCCGTGCGTCTGCTGC 1921 ggcttcattctgcatggttgtgcttaccagaccggagatattgacatcatatatgccttg 1980 CCGAAGTAAGACGTACCAACACGAATGGTCTGGCCTCTATAACTGTAGTATATACGGAAC 1981 agcaactgatagctgtcgctgtcaactgtcactgtaatacgctgcttcatagcatacctc 2040 TCGTTGACTATCGACAGCGACAGTTGACAGTGACATTATGCGACGAAGTATCGTATGGAG 2041 tttttgacatacttcgggtatacatatcagtatatattcttataccgcaaaaatcagcgc 2100 AAAAACTGTATGAAGCCCATATGTATAGTCATATATAAGAATATGGCGTTTTTAGTCGCG ORF_2 rf(4)(2150,2809)<<< | CAT marker(2150,2809)<<< | 2101 gcaaatacgcatactgttatctggcttttagtaagccggatccagatctttacgccccgc 2160 CGTTTATGCGTATGACAATAGACCGAAAATCATTCGGCCTAGGTCTAGAAATGCGGGGCG 2161 cctgccactcatcgcagtactgttgtaattcattaagcattctgccgacatggaagccat 2220 GGACGGTGAGTAGCGTCATGACAACATTAAGTAATTCGTAAGACGGCTGTACCTTCGGTA 2221 cacaaacggcatgatgaacctgaatcgccagcggcatcagcaccttgtcgccttgcgtat 2280 GTGTTTGCCGTACTACTTGGACTTAGCGGTCGCCGTAGTCGTGGAACAGCGGAACGCATA 2281 aatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacgtttaaat 2340 TTATAAACGGGTACCACTTTTGCCCCCGCTTCTTCAACAGGTATAACCGGTGCAAATTTA 2341 caaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaacc 2400 GTTTTGACCACTTTGAGTGGGTCCCTAACCGACTCTGCTTTTTGTATAAGAGTTATTTGG 2401 ctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgta 2460 GAAATCCCTTTATCCGGTCCAAAAGTGGCATTGTGCGGTGTAGAACGCTTATATACACAT 2461 gaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgct 2520 CTTTGACGGCCTTTAGCAGCACCATAAGTGAGGTCTCGCTACTTTTGCAAAGTCAAACGA 2521 catggaaaacggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttca 2580 GTACCTTTTGCCACATTGTTCCCACTTGTGATAGGGTATAGTGGTCGAGTGGCAGAAAGT EcoRI | 2581 ttgccatacggaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccg 2640 AACGGTATGCCTTAAGGCCTACTCGTAAGTAGTCCGCCCGTTCTTACACTTATTTCCGGC 2641 gataaaacttgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaa 2700 CTATTTTGAACACGAATAAAAAGAAATGCCAGAAATTTTTCCGGCATTATAGGTCGACTT 2701 cggtctggttataggtacattgagcaactgactgaaatgcctcaaaatgttctttacgat 2760 GCCAGACCAATATCCATGTAACTCGTTGACTGACTTTACGGAGTTTTACAAGAAATGCTA 2761 gccattgggatatatcaacggtggtatatccagtgatttttttctccattttagcttcct 2820 CGGTAACCCTATATAGTTGCCACCATATAGGTCACTAAAAAAAGAGGTAAAATCGAAGGA 2821 tagctcctgaaaatctcgacggatcctaactcaaaatccacacattatacgagccggaag 2880 ATCGAGGACTTTTAGAGCTGCCTAGGATTGAGTTTTAGGTGTGTAATATGCTCGGCCTTC attR1 other(2918,3042)<<< | NotI attR2 other(2918,3018)>>> | | 2881 cataaagtgtaaagcctggggtgcctaatgcggccgccatagtgactggatatgttgtgt 2940 GTATTTCACATTTCGGACCCCACGGATTACGCCGGCGGTATCACTGACCTATACAACACA 2941 tttacagtattatgtagtctgttttttatgcaaaatctaatttaatatattgatatttat 3000 AAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATA 3001 atcattttacgtttctcgttcagcttttttgtacaaacttgttgatatcgctagccaatt 3060 TAGTAAAATGCAAAGAGCAAGTCGAAAAAACATGTTTGAACAACTATAGCGATCGGTTAA 3061 gataacttcgtataggatactttatacgaagttatcattgggattcttcctattttgatc 3120 CTATTGAAGCATATCCTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAG loxP other(3166,3199)>>> | 3121 caagcatcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagc 3180 GTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCG XbaI | 3181 atacattatacgaagttatatggtgagcaagggcgattctagaaacttgtttattgcagc 3240 TATGTAATATGCTTCAATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCG 3241 ttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttc 3300 AATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAG HindIII | 3301 actgcattctagttgtggtttgtccaaactcatcaatgtatcttaaagcttaggaacccc 3360 TGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAATCCTTGGGG 3361 tagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgac 3420 ATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTG 3421 caaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgca 3480 GTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGT 3481 gggatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctc 3540 CCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAG 3541 ttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatc 3600 AAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAG 3601 agctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaa 3660 TCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTT pBR322 origin(3706,4325)<<< | 3661 catgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtt 3720 GTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAA 3721 tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg 3780 AAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCAC 3781 gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg 3840 CGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGC 3841 ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaag 3900 GAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTC 3901 cgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctc 3960 GCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAG 3961 caagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa 4020 GTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATT 4021 ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactgg 4080 GATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACC 4081 taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcc 4140 ATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGG 4141 taactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttac 4200 ATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATG 4201 cttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgg 4260 GAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACC 4261 tttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttt 4320 AAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAA 4321 gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt 4380 CTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCA 4381 catgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaa 4440 GTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATT 4441 atcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtga 4500 TAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACT 4501 ggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgt 4560 CCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCA 4561 gtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcg 4620 CATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGC 4621 agacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccga 4680 TCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCT 4681 gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggga 4740 CGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCT 4741 agctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttc 4800 TCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAG 4801 acctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaat 4860 TGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTA 4861 gtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagct 4920 CAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGA NEOKAN prom(4978,5027)>>> | 4921 tgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccg 4980 ACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGC 4981 gaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatg 5040 CTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTAC BclI | 5041 gctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacagga 5100 CGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCT NTP_II marker(5119,5907)>>> | ORF_1 rf(1)(5116,5910)>>> | | 5101 tgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgg 5160 ACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACC 5161 gtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgcc 5220 CACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGG 5221 gtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggt 5280 CACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCA 5281 gccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgtt 5340 CGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAA 5341 ccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggc 5400 GGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCG 5401 gaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatc 5460 CTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAG 5461 atggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccac 5520 TACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTG 5521 caagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcag 5580 GTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTC 5581 gatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaag 5640 CTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTC SphI | 5641 gcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaat 5700 CGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTA 5701 atcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcg 5760 TAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGC 5761 gaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaa 5820 CTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTT 5821 tgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgcc 5880 ACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGG 5881 ttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctc 5940 AAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAG 5941 attttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgc 6000 TAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCG f1 origin(6015,6320)<<< | 6001 gatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactc 6060 CTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAG 6061 caacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacc 6120 GTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGG 6121 caaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggag 6180 GTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTC 6181 cccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaa 6240 GGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTT 6241 agcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccac 6300 TCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTG 6301 cacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaat 6360 GTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTA 6361 taattcttaattaacatcatcaataatatacctt 6394 ATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 6394 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" Other Gene 1343..1462 /gene="attR1 other" Other Gene 1362..1462 /gene="attR2 other" misc_binding 1469..1474 /dbxref="REBASE:PstI" Other Gene 1503..1808 /gene="ccdB other" Marker 2150..2809 /gene="CAT marker" ORF 2150..2809 /sequence="ORF_2 rf(4)" misc_binding 2591..2596 /dbxref="REBASE:EcoRI" misc_binding 2910..2917 /dbxref="REBASE:NotI" Other Gene 2918..3042 /gene="attR1 other" Other Gene 2918..3018 /gene="attR2 other" Other Gene 3166..3199 /gene="loxP other" misc_binding 3218..3223 /dbxref="REBASE:XbaI" misc_binding 3346..3351 /dbxref="REBASE:HindIII" Rep_Origin 3706..4325 /gene="pBR322 origin" Promoter 4978..5027 /gene="NEOKAN prom" misc_binding 5085..5090 /dbxref="REBASE:BclI" ORF 5116..5910 /sequence="ORF_1 rf(1)" Marker 5119..5907 /gene="NTP_II marker" misc_binding 5645..5650 /dbxref="REBASE:SphI" Rep_Origin 6015..6320 /gene="f1 origin" BASE COUNT 1641 a 1569 c 1587 g 1597 t 0 others