pAAV-CMV-DIO-GFP-SV40-Puro
| Vector Name | pAAV-CMV-DIO-GFP-SV40-Puro |
| VectorType | AAV Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg 1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg 1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca 1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct 1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg 1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg 1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca 1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca 1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg 1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc 1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct 1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca 2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt 2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag 2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg 2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag 2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa 2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2401 tggtttgtcc aaactcatca atgtatctta aagcttggtg tggaaagtcc ccaggctccc 2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 2521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 2641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 2701 agctattcca gaagtagtga ggaggctttt ttggaggcca tgaccgagta caagcccacg 2761 gtgcgcctcg ccacccgcga cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc 2821 gccgactacc ccgccacgcg ccacaccgtg gacccggacc gccacatcga gcgggtcacc 2881 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 2941 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 3001 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 3061 cagatggaag ggctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 3121 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 3181 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 3241 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 3301 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac tgtgccttct agttgccagc 3361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 3421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 3481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg 3541 ctggggaaag cttaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 3601 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 3661 tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga tgcgtaagga 3721 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3781 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3841 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3901 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 3961 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4021 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4081 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4141 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4201 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4261 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4321 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4381 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4441 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4501 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4561 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4621 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4681 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4741 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4801 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4861 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4921 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 4981 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa 5041 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag 5101 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt 5161 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa 5221 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc 5281 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 5341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 5401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 5461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 5521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 5581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 5641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 5701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 5761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 5821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 5881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 5941 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 6001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 6061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt 6121 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt 6181 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc 6241 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 6301 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc 6361 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 6421 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 6481 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc 6541 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt
LOCUS dna 6596 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" ORF 1301..2203 /sequence="ORF_2 rf(2)" misc_binding 1322..1327 /dbxref="REBASE:XhoI" ORF 1352..2110 /sequence="ORF_4 rf(5)" misc_binding 1521..1526 /dbxref="REBASE:ApaI" misc_binding 1532..1537 /dbxref="REBASE:PstI" misc_binding 2233..2238 /dbxref="REBASE:BglII" Other Gene 2251..2284 /gene="loxP other" misc_binding 2303..2308 /dbxref="REBASE:XbaI" Promoter 2437..2705 /gene="SV40 prom" Rep_Origin 2604..2681 /gene="SV40 origin" ORF 2671..3339 /sequence="ORF_1 rf(1)" misc_binding 2684..2696 /dbxref="REBASE:SfiI" Marker 2740..3339 /gene="puro marker" misc_binding 3684..3689 /dbxref="REBASE:BamHI" Rep_Origin 3908..4527 /gene="pBR322 origin" Promoter 5180..5229 /gene="NEOKAN prom" misc_binding 5287..5292 /dbxref="REBASE:BclI" ORF 5318..6112 /sequence="ORF_3 rf(2)" Marker 5321..6109 /gene="NTP_II marker" Rep_Origin 6217..6522 /gene="f1 origin" BASE COUNT 1482 a 1778 c 1864 g 1472 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg 1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg 1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca 1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct 1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg 1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg 1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca 1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca 1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg 1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc 1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct 1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca 2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt 2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag 2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg 2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag 2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa 2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 2401 tggtttgtcc aaactcatca atgtatctta aagcttggtg tggaaagtcc ccaggctccc 2461 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 2521 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2581 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 2641 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 2701 agctattcca gaagtagtga ggaggctttt ttggaggcca tgaccgagta caagcccacg 2761 gtgcgcctcg ccacccgcga cgacgtccct cgggccgtac gcaccctcgc cgccgcgttc 2821 gccgactacc ccgccacgcg ccacaccgtg gacccggacc gccacatcga gcgggtcacc 2881 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 2941 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 3001 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 3061 cagatggaag ggctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 3121 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 3181 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac 3241 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 3301 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac tgtgccttct agttgccagc 3361 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 3421 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 3481 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg 3541 ctggggaaag cttaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 3601 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 3661 tcagtgagcg agcgagcgcg cagggatccc ggtgtgaaat accgcacaga tgcgtaagga 3721 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 3781 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 3841 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 3901 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 3961 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4021 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4081 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4141 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4201 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4261 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4321 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4381 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 4441 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 4501 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 4561 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 4621 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 4681 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 4741 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 4801 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 4861 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 4921 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 4981 acgttgttga aaaaggatct tcacctagat ccttttcacg tagaaagcca gtccgcagaa 5041 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag 5101 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt 5161 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa 5221 gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc gcaggggatc 5281 aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 5341 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 5401 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 5461 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 5521 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 5581 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 5641 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 5701 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 5761 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 5821 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 5881 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 5941 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 6001 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 6061 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaattttgtt 6121 aaaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aacatccctt 6181 ataaatcaaa agaatagacc gcgatagggt tgagtgttgt tccagtttgg aacaagagtc 6241 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 6301 gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc 6361 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 6421 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 6481 cggtcacgct gcgcgtaacc accacacccg cgcgcttaat gcgccgctac agggcgcgtc 6541 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata tacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG 481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540 TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC 541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600 TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT 601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660 TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC 661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720 GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA 721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780 CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT NdeI | 781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840 TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG 841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900 TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT 901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960 GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG 961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC 1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT KpnI | 1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200 GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC 1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260 ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG ORF_2 rf(2)(1301,2203)>>> | loxP other(1288,1321)<<< | | 1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320 GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT XhoI ORF_4 rf(5)(1352,2110)<<< | | 1321 tctcgagCAAGTTTGTACAAAAAAGCAGGCTtcagcgagatccggtggagccgggtccgg 1380 AGAGCTCGTTCAAACATGTTTTTTCGTCCGAAGTCGCTCTAGGCCACCTCGGCCCAGGCC 1381 cggtgccgtccacggcagaattggacgactgagcgcgggatctggcgaaggcgatggggg 1440 GCCACGGCAGGTGCCGTCTTAACCTGCTGACTCGCGCCCTAGACCGCTTCCGCTACCCCC 1441 tcttgaaggcgtgctggtactccacgatgcccagctcggtgttgctgtgcagctcctcca 1500 AGAACTTCCGCACGACCATGAGGTGCTACGGGTCGAGCCACAACGACACGTCGAGGAGGT ApaI PstI | | 1501 cgcggcggaaggcgaacatggggcccccgttctgcaggatgctggggtggatggcgctct 1560 GCGCCGCCTTCCGCTTGTACCCCGGGGGCAAGACGTCCTACGACCCCACCTACCGCGAGA 1561 tgaagtgcatgtggctgtccaccacgaagctgtagtagccgccgtcgcgcaggctgaagg 1620 ACTTCACGTACACCGACAGGTGGTGCTTCGACATCATCGGCGGCAGCGCGTCCGACTTCC 1621 tgcgggcgaagctgcccaccagcacgttatcgcccatggggtgcaggtgctccacggtgg 1680 ACGCCCGCTTCGACGGGTGGTCGTGCAATAGCGGGTACCCCACGTCCACGAGGTGCCACC 1681 cgttgctgcggatgatcttgtcggtgaagatcacgctgtcctcggggaagccggtgccca 1740 GCAACGACGCCTACTAGAACAGCCACTTCTAGTGCGACAGGAGCCCCTTCGGCCACGGGT 1741 ccaccttgaagtcgccgatcacgcggccggcctcgtagcggtagctgaagctcacgtgca 1800 GGTGGAACTTCAGCGGCTAGTGCGCCGGCCGGAGCATCGCCATCGACTTCGAGTGCACGT 1801 gcacgccgccgtcctcgtacttctcgatgcgggtgttggtgtagccgccgttgttgatgg 1860 CGTGCGGCGGCAGGAGCATGAAGAGCTACGCCCACAACCACATCGGCGGCAACAACTACC 1861 cgtgcaggaaggggttctcgtagccgctggggtaggtgccgaagtggtagaagccgtagc 1920 GCACGTCCTTCCCCAAGAGCATCGGCGACCCCATCCACGGCTTCACCATCTTCGGCATCG 1921 ccatcacgtggctcagcaggtaggggctgaaggtcagggcgcctttggtgctcttcatct 1980 GGTAGTGCACCGAGTCGTCCATCCCCGACTTCCAGTCCCGCGGAAACCACGAGAAGTAGA 1981 tgttggtcatgcggccctgcttgggggtgccctctccgccgcccaccagctcgaactcca 2040 ACAACCAGTACGCCGGGACGAACCCCCACGGGAGAGGCGGCGGGTGGTCGAGCTTGAGGT 2041 cgccgttcagggtgccggtgatgcggcactcgatctccatggcgggcaggccgctctcgt 2100 GCGGCAAGTCCCACGGCCACTACGCCGTGAGCTAGAGGTACCGCCCGTCCGGCGAGAGCA 2101 cgctctccaTACCCAGCTTTCTTGTACAAAGTGGgctagccaattgataacttcgtatag 2160 GCGAGAGGTATGGGTCGAAAGAACATGTTTCACCCGATCGGTTAACTATTGAAGCATATC 2161 gatactttatacgaagttatcattgggattcttcctattttgatccaagcatcaccatcg 2220 CTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGC BglII loxP other(2251,2284)>>> | | 2221 accctctagtccagatctcaccatcgacccataacttcgtatagcatacattatacgaag 2280 TGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTC XbaI | 2281 ttatatggtgagcaagggcgattctagaaacttgtttattgcagcttataatggttacaa 2340 AATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCGAATATTACCAATGTT 2341 ataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttg 2400 TATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAAC SV40 prom(2437,2705)>>> | 2401 tggtttgtccaaactcatcaatgtatcttaaagcttggtgtggaaagtccccaggctccc 2460 ACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAACCACACCTTTCAGGGGTCCGAGGG 2461 cagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagt 2520 GTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCA 2521 ccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaacca 2580 GGGGTCCGAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGT SV40 origin(2604,2681)>>> | 2581 tagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctc 2640 ATCAGGGCGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAG SfiI | ORF_1 rf(1)(2671,3339)>>> | | 2641 cgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctg 2700 GCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGAC puro marker(2740,3339)>>> | 2701 agctattccagaagtagtgaggaggcttttttggaggccatgaccgagtacaagcccacg 2760 TCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGC 2761 gtgcgcctcgccacccgcgacgacgtccctcgggccgtacgcaccctcgccgccgcgttc 2820 CACGCGGAGCGGTGGGCGCTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAG 2821 gccgactaccccgccacgcgccacaccgtggacccggaccgccacatcgagcgggtcacc 2880 CGGCTGATGGGGCGGTGCGCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGG 2881 gagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcg 2940 CTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGC 2941 gacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtg 3000 CTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCAC 3001 ttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaa 3060 AAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTT 3061 cagatggaagggctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccacc 3120 GTCTACCTTCCCGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGG 3121 gtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccgga 3180 CAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCT 3181 gtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaac 3240 CACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTG 3241 ctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaagga 3300 GAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCT 3301 ccgcgcacctggtgcatgacccgcaagcccggtgcctgactgtgccttctagttgccagc 3360 GGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTGACACGGAAGATCAACGGTCG 3361 catctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactg 3420 GTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGAC 3421 tcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattc 3480 AGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAG 3481 tggggggtggggtggggcaggacagcaagggggaggattgggaagagaatagcaggcatg 3540 ACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTCTTATCGTCCGTAC 3541 ctggggaaagcttaggaacccctagtgatggagttggccactccctctctgcgcgctcgc 3600 GACCCCTTTCGAATCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCG 3601 tcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcc 3660 AGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGG BamHI | 3661 tcagtgagcgagcgagcgcgcagggatcccggtgtgaaataccgcacagatgcgtaagga 3720 AGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCT 3721 gaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcg 3780 CTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGC 3781 ttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaat 3840 AAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTA 3841 caggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgta 3900 GTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCAT pBR322 origin(3908,4527)<<< | 3901 aaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaa 3960 TTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTT 3961 atcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttc 4020 TAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAG 4021 cccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgt 4080 GGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACA 4081 ccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctca 4140 GGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGT 4141 gttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg 4200 CAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGC 4201 accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttat 4260 TGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATA 4261 cgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgcta 4320 GCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGAT 4321 cagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatct 4380 GTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGA 4381 gcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaac 4440 CGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTG 4441 aaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaa 4500 TTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTT 4501 aaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaa 4560 TTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTT 4561 actcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttt 4620 TGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAA 4621 taaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgaca 4680 ATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGT 4681 gttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcca 4740 CAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGT 4741 tagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcc 4800 ATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGG 4801 ccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataa 4860 GGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATT 4861 accagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatcc 4920 TGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGG 4921 agtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgca 4980 TCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGT 4981 acgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaa 5040 TGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTT 5041 acggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaag 5100 TGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTC 5101 cgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggt 5160 GCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCA NEOKAN prom(5180,5229)>>> | 5161 tttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaa 5220 AAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTT 5221 gccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatc 5280 CGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAG NTP_II marker(5321,6109)>>> | BclI ORF_3 rf(2)(5318,6112)>>> | | | 5281 aagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgca 5340 TTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGT 5341 cgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagac 5400 GCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTG 5401 aatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttt 5460 TTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAA 5461 tgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatc 5520 ACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAG 5521 gtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcggg 5580 CACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCC 5581 aagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgc 5640 TTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACG 5641 tcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatcc 5700 AGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGG 5701 ggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggat 5760 CCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTA 5761 ggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagc 5820 CCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCG 5821 cgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgaccca 5880 GCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGT 5881 tggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcga 5940 ACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCT 5941 ctgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatat 6000 GACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATA 6001 tgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgc 6060 ACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCG 6061 tcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgtt 6120 AGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAA 6121 aaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatccctt 6180 TTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAA f1 origin(6217,6522)<<< | 6181 ataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtc 6240 TATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAG 6241 cactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatg 6300 GTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTAC 6301 gcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctc 6360 CGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAG 6361 taaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacg 6420 ATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGC 6421 tggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtag 6480 ACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATC 6481 cggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtc 6540 GCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAG 6541 cattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 6596 GTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 6596 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" ORF 1301..2203 /sequence="ORF_2 rf(2)" misc_binding 1322..1327 /dbxref="REBASE:XhoI" ORF 1352..2110 /sequence="ORF_4 rf(5)" misc_binding 1521..1526 /dbxref="REBASE:ApaI" misc_binding 1532..1537 /dbxref="REBASE:PstI" misc_binding 2233..2238 /dbxref="REBASE:BglII" Other Gene 2251..2284 /gene="loxP other" misc_binding 2303..2308 /dbxref="REBASE:XbaI" Promoter 2437..2705 /gene="SV40 prom" Rep_Origin 2604..2681 /gene="SV40 origin" ORF 2671..3339 /sequence="ORF_1 rf(1)" misc_binding 2684..2696 /dbxref="REBASE:SfiI" Marker 2740..3339 /gene="puro marker" misc_binding 3684..3689 /dbxref="REBASE:BamHI" Rep_Origin 3908..4527 /gene="pBR322 origin" Promoter 5180..5229 /gene="NEOKAN prom" misc_binding 5287..5292 /dbxref="REBASE:BclI" ORF 5318..6112 /sequence="ORF_3 rf(2)" Marker 5321..6109 /gene="NTP_II marker" Rep_Origin 6217..6522 /gene="f1 origin" BASE COUNT 1482 a 1778 c 1864 g 1472 t 0 others