pAAV-CMV-DIO-GFP
| Vector Name | pAAV-CMV-DIO-GFP |
| VectorType | AAV Vector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg
1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg
1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca
1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct
1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg
1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg
1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca
1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca
1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg
1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc
1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct
1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca
2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt
2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag
2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg
2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag
2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa
2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
2401 tggtttgtcc aaactcatca atgtatctta aagcttagga acccctagtg atggagttgg
2461 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
2521 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagggat cccggtgtga
2581 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
2641 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
2701 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
2761 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
2821 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
2881 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
2941 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3001 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3061 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
3121 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
3181 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
3241 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
3301 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
3361 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3421 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
3481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
3541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
3601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
3661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
3721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
3781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
3841 ttcgccagtt aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc
3901 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
3961 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
4021 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
4081 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca
4141 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
4201 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
4261 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
4321 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
4381 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
4441 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
4501 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
4561 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
4621 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
4681 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
4741 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
4801 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
4861 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
4921 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
4981 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata
5041 ggccgaaatc ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt
5101 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
5161 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt
5221 gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc
5281 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
5341 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt
5401 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac
5461 atcatcaata atatacctt
LOCUS dna 5479 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
ORF 1301..2203
/sequence="ORF_2 rf(2)"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
ORF 1352..2110
/sequence="ORF_3 rf(4)"
misc_binding 1521..1526
/dbxref="REBASE:ApaI"
misc_binding 1532..1537
/dbxref="REBASE:PstI"
misc_binding 2233..2238
/dbxref="REBASE:BglII"
Other Gene 2251..2284
/gene="loxP other"
misc_binding 2303..2308
/dbxref="REBASE:XbaI"
misc_binding 2431..2436
/dbxref="REBASE:HindIII"
misc_binding 2567..2572
/dbxref="REBASE:BamHI"
Rep_Origin 2791..3410
/gene="pBR322 origin"
Promoter 4063..4112
/gene="NEOKAN prom"
misc_binding 4170..4175
/dbxref="REBASE:BclI"
ORF 4201..4995
/sequence="ORF_1 rf(1)"
Marker 4204..4992
/gene="NTP_II marker"
misc_binding 4730..4735
/dbxref="REBASE:SphI"
Rep_Origin 5100..5405
/gene="f1 origin"
BASE COUNT 1284 a 1398 c 1521 g 1276 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc
481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta
601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg
661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc
961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg
1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc
1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta
1321 tctcgagcaa gtttgtacaa aaaagcaggc ttcagcgaga tccggtggag ccgggtccgg
1381 cggtgccgtc cacggcagaa ttggacgact gagcgcggga tctggcgaag gcgatggggg
1441 tcttgaaggc gtgctggtac tccacgatgc ccagctcggt gttgctgtgc agctcctcca
1501 cgcggcggaa ggcgaacatg gggcccccgt tctgcaggat gctggggtgg atggcgctct
1561 tgaagtgcat gtggctgtcc accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg
1621 tgcgggcgaa gctgcccacc agcacgttat cgcccatggg gtgcaggtgc tccacggtgg
1681 cgttgctgcg gatgatcttg tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca
1741 ccaccttgaa gtcgccgatc acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca
1801 gcacgccgcc gtcctcgtac ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg
1861 cgtgcaggaa ggggttctcg tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc
1921 ccatcacgtg gctcagcagg taggggctga aggtcagggc gcctttggtg ctcttcatct
1981 tgttggtcat gcggccctgc ttgggggtgc cctctccgcc gcccaccagc tcgaactcca
2041 cgccgttcag ggtgccggtg atgcggcact cgatctccat ggcgggcagg ccgctctcgt
2101 cgctctccat acccagcttt cttgtacaaa gtgggctagc caattgataa cttcgtatag
2161 gatactttat acgaagttat cattgggatt cttcctattt tgatccaagc atcaccatcg
2221 accctctagt ccagatctca ccatcgaccc ataacttcgt atagcataca ttatacgaag
2281 ttatatggtg agcaagggcg attctagaaa cttgtttatt gcagcttata atggttacaa
2341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
2401 tggtttgtcc aaactcatca atgtatctta aagcttagga acccctagtg atggagttgg
2461 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
2521 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagggat cccggtgtga
2581 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
2641 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
2701 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
2761 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
2821 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
2881 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
2941 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
3001 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
3061 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
3121 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
3181 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
3241 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
3301 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
3361 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
3421 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
3481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
3541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
3601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
3661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
3721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
3781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
3841 ttcgccagtt aatagtttgc gcaacgttgt tgaaaaagga tcttcaccta gatccttttc
3901 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
3961 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
4021 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
4081 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt ctcgccgcca
4141 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
4201 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
4261 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
4321 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
4381 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
4441 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
4501 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
4561 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
4621 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
4681 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
4741 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
4801 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
4861 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
4921 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
4981 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata
5041 ggccgaaatc ggcaacatcc cttataaatc aaaagaatag accgcgatag ggttgagtgt
5101 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
5161 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat caagtttttt
5221 gcggtcgagg tgccgtaaag ctctaaatcg gaaccctaaa gggagccccc gatttagagc
5281 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
5341 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgcgcgctt
5401 aatgcgccgc tacagggcgc gtccattcgc cattcaggat cgaattaatt cttaattaac
5461 atcatcaata atatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
HincII
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG
481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540
TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC
541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600
TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT
601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660
TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC
661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720
GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA
721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780
CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT
NdeI
|
781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840
TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG
841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900
TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT
901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960
GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG
961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020
TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT
1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080
AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC
1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140
CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT
KpnI
|
1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200
GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC
1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260
ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG
ORF_2 rf(2)(1301,2203)>>>
|
loxP other(1288,1321)<<<
| |
1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320
GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT
XhoI ORF_3 rf(4)(1352,2110)<<<
| |
1321 tctcgagCAAGTTTGTACAAAAAAGCAGGCTtcagcgagatccggtggagccgggtccgg 1380
AGAGCTCGTTCAAACATGTTTTTTCGTCCGAAGTCGCTCTAGGCCACCTCGGCCCAGGCC
1381 cggtgccgtccacggcagaattggacgactgagcgcgggatctggcgaaggcgatggggg 1440
GCCACGGCAGGTGCCGTCTTAACCTGCTGACTCGCGCCCTAGACCGCTTCCGCTACCCCC
1441 tcttgaaggcgtgctggtactccacgatgcccagctcggtgttgctgtgcagctcctcca 1500
AGAACTTCCGCACGACCATGAGGTGCTACGGGTCGAGCCACAACGACACGTCGAGGAGGT
ApaI PstI
| |
1501 cgcggcggaaggcgaacatggggcccccgttctgcaggatgctggggtggatggcgctct 1560
GCGCCGCCTTCCGCTTGTACCCCGGGGGCAAGACGTCCTACGACCCCACCTACCGCGAGA
1561 tgaagtgcatgtggctgtccaccacgaagctgtagtagccgccgtcgcgcaggctgaagg 1620
ACTTCACGTACACCGACAGGTGGTGCTTCGACATCATCGGCGGCAGCGCGTCCGACTTCC
1621 tgcgggcgaagctgcccaccagcacgttatcgcccatggggtgcaggtgctccacggtgg 1680
ACGCCCGCTTCGACGGGTGGTCGTGCAATAGCGGGTACCCCACGTCCACGAGGTGCCACC
1681 cgttgctgcggatgatcttgtcggtgaagatcacgctgtcctcggggaagccggtgccca 1740
GCAACGACGCCTACTAGAACAGCCACTTCTAGTGCGACAGGAGCCCCTTCGGCCACGGGT
1741 ccaccttgaagtcgccgatcacgcggccggcctcgtagcggtagctgaagctcacgtgca 1800
GGTGGAACTTCAGCGGCTAGTGCGCCGGCCGGAGCATCGCCATCGACTTCGAGTGCACGT
1801 gcacgccgccgtcctcgtacttctcgatgcgggtgttggtgtagccgccgttgttgatgg 1860
CGTGCGGCGGCAGGAGCATGAAGAGCTACGCCCACAACCACATCGGCGGCAACAACTACC
1861 cgtgcaggaaggggttctcgtagccgctggggtaggtgccgaagtggtagaagccgtagc 1920
GCACGTCCTTCCCCAAGAGCATCGGCGACCCCATCCACGGCTTCACCATCTTCGGCATCG
1921 ccatcacgtggctcagcaggtaggggctgaaggtcagggcgcctttggtgctcttcatct 1980
GGTAGTGCACCGAGTCGTCCATCCCCGACTTCCAGTCCCGCGGAAACCACGAGAAGTAGA
1981 tgttggtcatgcggccctgcttgggggtgccctctccgccgcccaccagctcgaactcca 2040
ACAACCAGTACGCCGGGACGAACCCCCACGGGAGAGGCGGCGGGTGGTCGAGCTTGAGGT
2041 cgccgttcagggtgccggtgatgcggcactcgatctccatggcgggcaggccgctctcgt 2100
GCGGCAAGTCCCACGGCCACTACGCCGTGAGCTAGAGGTACCGCCCGTCCGGCGAGAGCA
2101 cgctctccaTACCCAGCTTTCTTGTACAAAGTGGgctagccaattgataacttcgtatag 2160
GCGAGAGGTATGGGTCGAAAGAACATGTTTCACCCGATCGGTTAACTATTGAAGCATATC
2161 gatactttatacgaagttatcattgggattcttcctattttgatccaagcatcaccatcg 2220
CTATGAAATATGCTTCAATAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGC
BglII loxP other(2251,2284)>>>
| |
2221 accctctagtccagatctcaccatcgacccataacttcgtatagcatacattatacgaag 2280
TGGGAGATCAGGTCTAGAGTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTC
XbaI
|
2281 ttatatggtgagcaagggcgattctagaaacttgtttattgcagcttataatggttacaa 2340
AATATACCACTCGTTCCCGCTAAGATCTTTGAACAAATAACGTCGAATATTACCAATGTT
2341 ataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttg 2400
TATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAAC
HindIII
|
2401 tggtttgtccaaactcatcaatgtatcttaaagcttaggaacccctagtgatggagttgg 2460
ACCAAACAGGTTTGAGTAGTTACATAGAATTTCGAATCCTTGGGGATCACTACCTCAACC
2461 ccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgac 2520
GGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTG
BamHI
|
2521 gcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagggatcccggtgtga 2580
CGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCACACT
2581 aataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgct 2640
TTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGA
2641 cactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggc 2700
GTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCG
2701 ggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaagg 2760
CCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCC
pBR322 origin(2791,3410)<<<
|
2761 ccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccg 2820
GGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGC
2821 cccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagg 2880
GGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCC
2881 actataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgac 2940
TGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTG
2941 cctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctca 3000
GGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGT
3001 tagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgt 3060
ATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACA
3061 gcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtc 3120
CGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAG
3121 caacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcag 3180
GTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTC
3181 agcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac 3240
TCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTG
3241 tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagt 3300
ATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCA
3301 tggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaa 3360
ACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTT
3361 gcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggg 3420
CGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCC
3421 gtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaa 3480
CAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTT
3481 aaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtat 3540
TTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATA
3541 atatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagc 3600
TATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCG
3601 gatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgat 3660
CTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTA
3661 acgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcacc 3720
TGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGG
3721 ggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcc 3780
CCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGG
3781 tgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtag 3840
ACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATC
3841 ttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttc 3900
AAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAG
3901 acgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgggct 3960
TGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGA
3961 atctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttaca 4020
TAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGT
NEOKAN prom(4063,4112)>>>
|
4021 tggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagctggg 4080
ACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCC
4081 gcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgccgcca 4140
CGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGT
BclI
|
4141 aggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtttcgc 4200
TCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCG
NTP_II marker(4204,4992)>>>
|
ORF_1 rf(1)(4201,4995)>>>
| |
4201 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattc 4260
TACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAG
4261 ggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtca 4320
CCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGT
4321 gcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactg 4380
CGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGAC
4381 caagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtg 4440
GTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACAC
4441 ctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcag 4500
GAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTC
4501 gatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatg 4560
CTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTAC
4561 cggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgc 4620
GCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCG
4621 atcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaa 4680
TAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTT
SphI
|
4681 gagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgac 4740
CTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTG
4741 ggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaat 4800
CCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTA
4801 ggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggac 4860
CCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTG
4861 atagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttc 4920
TATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAG
4921 ctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttctt 4980
GAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAA
4981 gacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaaccaata 5040
CTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTAT
5041 ggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttgagtgt 5100
CCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACA
f1 origin(5100,5405)<<<
|
5101 tgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcg 5160
ACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGC
5161 aaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagtttttt 5220
TTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAA
5221 gcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgatttagagc 5280
CGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCG
5281 ttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcggg 5340
AACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCC
5341 cgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgctt 5400
GCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAA
5401 aatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaattaac 5460
TTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTG
5461 atcatcaataatatacctt 5479
TAGTAGTTATTATATGGAA
LOCUS dna 5479 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 457..462
/dbxref="REBASE:HincII"
misc_binding 833..838
/dbxref="REBASE:NdeI"
misc_binding 1168..1173
/dbxref="REBASE:KpnI"
Other Gene 1288..1321
/gene="loxP other"
ORF 1301..2203
/sequence="ORF_2 rf(2)"
misc_binding 1322..1327
/dbxref="REBASE:XhoI"
ORF 1352..2110
/sequence="ORF_3 rf(4)"
misc_binding 1521..1526
/dbxref="REBASE:ApaI"
misc_binding 1532..1537
/dbxref="REBASE:PstI"
misc_binding 2233..2238
/dbxref="REBASE:BglII"
Other Gene 2251..2284
/gene="loxP other"
misc_binding 2303..2308
/dbxref="REBASE:XbaI"
misc_binding 2431..2436
/dbxref="REBASE:HindIII"
misc_binding 2567..2572
/dbxref="REBASE:BamHI"
Rep_Origin 2791..3410
/gene="pBR322 origin"
Promoter 4063..4112
/gene="NEOKAN prom"
misc_binding 4170..4175
/dbxref="REBASE:BclI"
ORF 4201..4995
/sequence="ORF_1 rf(1)"
Marker 4204..4992
/gene="NTP_II marker"
misc_binding 4730..4735
/dbxref="REBASE:SphI"
Rep_Origin 5100..5405
/gene="f1 origin"
BASE COUNT 1284 a 1398 c 1521 g 1276 t 0 others