pAAV-CMV-DIO-Blank-SV40-Puro
Vector Name | pAAV-CMV-DIO-Blank-SV40-Puro |
VectorType | AAV Vector |
Antibiotic Information | Bacterial: KanamycinMammalian: Puromycin |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt atacgaagtt 1381 atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta gtccagatct 1441 caccatcgac ccataacttc gtatagcata cattatacga agttatatgg tgagcaaggg 1501 cgattctaga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 1621 caatgtatct taaagcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 1681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 1921 gaggaggctt ttttggaggc catgaccgag tacaagccca cggtgcgcct cgccacccgc 1981 gacgacgtcc ctcgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 2041 cgccacaccg tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 2101 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 2161 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 2221 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga agggctcctg 2281 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 2341 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 2401 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 2461 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 2521 acccgcaagc ccggtgcctg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2701 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggaa agcttaggaa 2761 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 2821 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 2881 cgcagggatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 2941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat 4201 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat 4261 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt 4321 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga 4381 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg 4441 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac 4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5281 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca 5341 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga 5401 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 5461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 5521 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag 5581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 5641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 5701 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc 5761 gaattaattc ttaattaaca tcatcaataa tatacctt
LOCUS dna 5798 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" misc_binding 1331..1336 /dbxref="REBASE:EcoRV" misc_binding 1435..1440 /dbxref="REBASE:BglII" Other Gene 1453..1486 /gene="loxP other" misc_binding 1505..1510 /dbxref="REBASE:XbaI" Promoter 1639..1907 /gene="SV40 prom" Rep_Origin 1806..1883 /gene="SV40 origin" ORF 1873..2541 /sequence="ORF_1 rf(1)" misc_binding 1886..1898 /dbxref="REBASE:SfiI" Marker 1942..2541 /gene="puro marker" misc_binding 2886..2891 /dbxref="REBASE:BamHI" Rep_Origin 3110..3729 /gene="pBR322 origin" Promoter 4382..4431 /gene="NEOKAN prom" misc_binding 4489..4494 /dbxref="REBASE:BclI" ORF 4520..5314 /sequence="ORF_2 rf(2)" Marker 4523..5311 /gene="NTP_II marker" Rep_Origin 5419..5724 /gene="f1 origin" BASE COUNT 1359 a 1547 c 1583 g 1309 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt atacgaagtt 1381 atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta gtccagatct 1441 caccatcgac ccataacttc gtatagcata cattatacga agttatatgg tgagcaaggg 1501 cgattctaga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 1621 caatgtatct taaagcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 1681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 1921 gaggaggctt ttttggaggc catgaccgag tacaagccca cggtgcgcct cgccacccgc 1981 gacgacgtcc ctcgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 2041 cgccacaccg tggacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 2101 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 2161 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 2221 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga agggctcctg 2281 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 2341 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 2401 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 2461 ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg 2521 acccgcaagc ccggtgcctg actgtgcctt ctagttgcca gccatctgtt gtttgcccct 2581 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 2641 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 2701 aggacagcaa gggggaggat tgggaagaga atagcaggca tgctggggaa agcttaggaa 2761 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg 2821 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg 2881 cgcagggatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 2941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat 4201 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat 4261 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt 4321 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga 4381 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg 4441 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac 4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5281 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca 5341 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga 5401 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 5461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 5521 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag 5581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 5641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 5701 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc 5761 gaattaattc ttaattaaca tcatcaataa tatacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG 481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540 TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC 541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600 TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT 601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660 TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC 661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720 GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA 721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780 CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT NdeI | 781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840 TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG 841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900 TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT 901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960 GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG 961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC 1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT KpnI | 1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200 GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC 1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260 ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG loxP other(1288,1321)<<< | 1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320 GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT XhoI EcoRV | | 1321 tctcgagtcagatatcgctagccaattgataacttcgtataggatactttatacgaagtt 1380 AGAGCTCAGTCTATAGCGATCGGTTAACTATTGAAGCATATCCTATGAAATATGCTTCAA BglII | 1381 atcattgggattcttcctattttgatccaagcatcaccatcgaccctctagtccagatct 1440 TAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGA loxP other(1453,1486)>>> | 1441 caccatcgacccataacttcgtatagcatacattatacgaagttatatggtgagcaaggg 1500 GTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTCAATATACCACTCGTTCCC XbaI | 1501 cgattctagaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcac 1560 GCTAAGATCTTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTG 1561 aaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcat 1620 TTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTA SV40 prom(1639,1907)>>> | 1621 caatgtatcttaaagcttggtgtggaaagtccccaggctccccagcaggcagaagtatgc 1680 GTTACATAGAATTTCGAACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACG 1681 aaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcag 1740 TTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTC 1741 gcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactc 1800 CGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAG SV40 origin(1806,1883)>>> | 1801 cgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaa 1860 GCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATT SfiI | ORF_1 rf(1)(1873,2541)>>> | | 1861 ttttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagt 1920 AAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCA puro marker(1942,2541)>>> | 1921 gaggaggcttttttggaggccatgaccgagtacaagcccacggtgcgcctcgccacccgc 1980 CTCCTCCGAAAAAACCTCCGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCG 1981 gacgacgtccctcgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacg 2040 CTGCTGCAGGGAGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGC 2041 cgccacaccgtggacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttc 2100 GCGGTGTGGCACCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAG 2101 ctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtg 2160 GAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCAC 2161 gcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccg 2220 CGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGC 2221 cgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaagggctcctg 2280 GCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCCGAGGAC 2281 gcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgac 2340 CGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTG 2341 caccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgc 2400 GTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCG 2401 gccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcgg 2460 CGGCCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCC 2461 ctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatg 2520 GAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTAC 2521 acccgcaagcccggtgcctgactgtgccttctagttgccagccatctgttgtttgcccct 2580 TGGGCGTTCGGGCCACGGACTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGA 2581 cccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatg 2640 GGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTAC 2641 aggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggc 2700 TCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCG 2701 aggacagcaagggggaggattgggaagagaatagcaggcatgctggggaaagcttaggaa 2760 TCCTGTCGTTCCCCCTCCTAACCCTTCTCTTATCGTCCGTACGACCCCTTTCGAATCCTT 2761 cccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccggg 2820 GGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCCC 2821 cgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcg 2880 GCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCGC BamHI | 2881 cgcagggatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggc 2940 GCGTCCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCG 2941 gctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg 3000 CGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCC 3001 tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa 3060 ATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTT pBR322 origin(3110,3729)<<< | 3061 agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgg 3120 TCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACC 3121 cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga 3180 GCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCT 3181 ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcg 3240 CCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGC 3241 tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg 3300 ACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCC 3301 gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttc 3360 CTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAG 3361 gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg 3420 CGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGC 3421 gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca 3480 CATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGT 3481 ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggt 3540 GACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCA 3541 ggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccag 3600 CCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTC 3601 ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg 3660 AATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGC 3661 gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc 3720 CACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAG 3721 ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt 3780 GAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAA 3781 tggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtt 3840 ACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAA 3841 ttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca 3900 AATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGT 3901 gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg 3960 CACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGC 3961 tcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatac 4020 AGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATG 4021 cgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaaggg 4080 GCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCC 4081 ccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc 4140 GGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGG 4141 gggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggat 4200 CCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTA 4201 cttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggat 4260 GAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTA 4261 gaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggt 4320 CTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCA 4321 agcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcga 4380 TCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCT NEOKAN prom(4382,4431)>>> | 4381 accggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactg 4440 TGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGAC BclI | 4441 gatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagac 4500 CTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTG NTP_II marker(4523,5311)>>> | ORF_2 rf(2)(4520,5314)>>> | | 4501 aggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgc 4560 TCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCG 4561 ttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgc 4620 AACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACG 4621 cgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtc 4680 GCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAG 4681 cggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacggg 4740 GCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCC 4741 cgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctatt 4800 GCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAA 4801 gggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatc 4860 CCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAG 4861 catcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcga 4920 GTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCT 4921 ccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcga 4980 GGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCT 4981 tcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggct 5040 AGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGA 5041 caaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgcc 5100 GTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGG 5101 gaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgt 5160 CTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACA 5161 ggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcgg 5220 CCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCC 5221 cgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcat 5280 GCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTA 5281 cgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatca 5340 GCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGT 5341 gctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaataga 5400 CGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCT f1 origin(5419,5724)<<< | 5401 ccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgg 5460 GGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACC 5461 actccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccat 5520 TGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTA 5521 cacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaag 5580 GTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTC 5581 ggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaaggga 5640 CCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCT 5641 agaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaa 5700 TCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATT 5701 ccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatc 5760 GGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAG 5761 gaattaattcttaattaacatcatcaataatatacctt 5798 CTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 5798 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" misc_binding 1331..1336 /dbxref="REBASE:EcoRV" misc_binding 1435..1440 /dbxref="REBASE:BglII" Other Gene 1453..1486 /gene="loxP other" misc_binding 1505..1510 /dbxref="REBASE:XbaI" Promoter 1639..1907 /gene="SV40 prom" Rep_Origin 1806..1883 /gene="SV40 origin" ORF 1873..2541 /sequence="ORF_1 rf(1)" misc_binding 1886..1898 /dbxref="REBASE:SfiI" Marker 1942..2541 /gene="puro marker" misc_binding 2886..2891 /dbxref="REBASE:BamHI" Rep_Origin 3110..3729 /gene="pBR322 origin" Promoter 4382..4431 /gene="NEOKAN prom" misc_binding 4489..4494 /dbxref="REBASE:BclI" ORF 4520..5314 /sequence="ORF_2 rf(2)" Marker 4523..5311 /gene="NTP_II marker" Rep_Origin 5419..5724 /gene="f1 origin" BASE COUNT 1359 a 1547 c 1583 g 1309 t 0 others