pAAV-CMV-DIO-Blank-SV40-GFP
Vector Name | pAAV-CMV-DIO-Blank-SV40-GFP |
VectorType | AAV Vector |
Antibiotic Information | Kanamycin |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt atacgaagtt 1381 atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta gtccagatct 1441 caccatcgac ccataacttc gtatagcata cattatacga agttatatgg tgagcaaggg 1501 cgattctaga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 1621 caatgtatct taaagcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 1681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 1921 gaggaggctt ttttggaggc cgccaccatg gagagcgacg agagcggcct gcccgccatg 1981 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 2041 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 2101 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 2161 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 2221 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 2281 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 2341 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 2401 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 2461 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 2521 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 2581 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 2641 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 2701 cgctgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 2761 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 2821 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 2881 aggattggga agagaatagc aggcatgctg gggaaagctt aggaacccct agtgatggag 2941 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 3001 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ggatcccggt 3061 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 3121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgaaaa aggatcttca cctagatcct 4381 tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg 4441 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct 4501 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc 4561 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttctcgcc 4621 gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt 4681 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 4741 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 4801 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 4861 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 4921 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 4981 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 5041 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5101 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5161 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 5221 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 5281 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 5341 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 5401 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 5461 tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca ttttttaacc 5521 aataggccga aatcggcaac atcccttata aatcaaaaga atagaccgcg atagggttga 5581 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 5641 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt 5701 ttttgcggtc gaggtgccgt aaagctctaa atcggaaccc taaagggagc ccccgattta 5761 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 5821 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgcgc 5881 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat 5941 taacatcatc aataatatac ctt
LOCUS dna 5963 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" misc_binding 1331..1336 /dbxref="REBASE:EcoRV" misc_binding 1435..1440 /dbxref="REBASE:BglII" Other Gene 1453..1486 /gene="loxP other" misc_binding 1505..1510 /dbxref="REBASE:XbaI" Promoter 1639..1907 /gene="SV40 prom" Rep_Origin 1806..1883 /gene="SV40 origin" ORF 1873..2706 /sequence="ORF_1 rf(1)" misc_binding 1886..1898 /dbxref="REBASE:SfiI" misc_binding 2521..2526 /dbxref="REBASE:PstI" misc_binding 2532..2537 /dbxref="REBASE:ApaI" misc_binding 3051..3056 /dbxref="REBASE:BamHI" Rep_Origin 3275..3894 /gene="pBR322 origin" Promoter 4547..4596 /gene="NEOKAN prom" misc_binding 4654..4659 /dbxref="REBASE:BclI" ORF 4685..5479 /sequence="ORF_2 rf(2)" Marker 4688..5476 /gene="NTP_II marker" Rep_Origin 5584..5889 /gene="f1 origin" BASE COUNT 1428 a 1590 c 1601 g 1344 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta acctgcgcgc tcgctcgctc 481 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg 541 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc cttgtagtta 601 atgattaacc cgccatgcta cttatctacg tagccatgct ctaggaagat cgcctaggcg 661 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 721 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 781 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 841 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 901 catgacctta tgggactttc ctacttggca gtacatctac gtttagtcat cgctattacc 961 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 1021 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 1081 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 1141 cggtgggagg tctatataag cagagctggt accaatcagc tcgctcacca tataacttcg 1201 tataaagtat cctatacgaa gttatatcaa aataggaaga ccaatgcttc accatcgacc 1261 cgaattgcca agcatcacca tcgacccata acttcgtata atgtatgcta tacgaagtta 1321 tctcgagtca gatatcgcta gccaattgat aacttcgtat aggatacttt atacgaagtt 1381 atcattggga ttcttcctat tttgatccaa gcatcaccat cgaccctcta gtccagatct 1441 caccatcgac ccataacttc gtatagcata cattatacga agttatatgg tgagcaaggg 1501 cgattctaga aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 1561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 1621 caatgtatct taaagcttgg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 1681 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 1741 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 1801 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 1861 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 1921 gaggaggctt ttttggaggc cgccaccatg gagagcgacg agagcggcct gcccgccatg 1981 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc 2041 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc 2101 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc 2161 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc 2221 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc 2281 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac 2341 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc 2401 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc 2461 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc 2521 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc 2581 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc 2641 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct 2701 cgctgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 2761 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 2821 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 2881 aggattggga agagaatagc aggcatgctg gggaaagctt aggaacccct agtgatggag 2941 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 3001 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ggatcccggt 3061 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 3121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 3361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 3421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 3481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 3541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 3601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 3661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 3721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 3781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 3841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 3901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 3961 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4021 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4081 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4141 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4201 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4261 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4321 gtagttcgcc agttaatagt ttgcgcaacg ttgttgaaaa aggatcttca cctagatcct 4381 tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg 4441 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct 4501 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc 4561 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttctcgcc 4621 gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt 4681 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 4741 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 4801 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 4861 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 4921 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 4981 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 5041 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5101 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5161 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc 5221 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 5281 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 5341 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 5401 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 5461 tcttgacgag ttcttctgaa ttttgttaaa atttttgtta aatcagctca ttttttaacc 5521 aataggccga aatcggcaac atcccttata aatcaaaaga atagaccgcg atagggttga 5581 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 5641 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc aaatcaagtt 5701 ttttgcggtc gaggtgccgt aaagctctaa atcggaaccc taaagggagc ccccgattta 5761 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 5821 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgcgc 5881 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat 5941 taacatcatc aataatatac ctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaacctgcgcgctcgctcgctc 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGGACGCGCGAGCGAGCGAG 481 actgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtg 540 TGACTCCGGCGGGCCCGTTTCGGGCCCGCAGCCCGCTGGAAACCAGCGGGCCGGAGTCAC 541 agcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagtta 600 TCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGATCCCCAAGGAACATCAAT 601 atgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgcctaggcg 660 TACTAATTGGGCGGTACGATGAATAGATGCATCGGTACGAGATCCTTCTAGCGGATCCGC 661 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 720 GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA 721 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 780 CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT NdeI | 781 atgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 840 TACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG 841 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 900 TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT 901 catgaccttatgggactttcctacttggcagtacatctacgtttagtcatcgctattacc 960 GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGG 961 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 1020 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 1021 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 1080 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC 1081 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 1140 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT KpnI | 1141 cggtgggaggtctatataagcagagctggtaccaatcagctcgctcaccatataacttcg 1200 GCCACCCTCCAGATATATTCGTCTCGACCATGGTTAGTCGAGCGAGTGGTATATTGAAGC 1201 tataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacc 1260 ATATTTCATAGGATATGCTTCAATATAGTTTTATCCTTCTGGTTACGAAGTGGTAGCTGG loxP other(1288,1321)<<< | 1261 cgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagtta 1320 GCTTAACGGTTCGTAGTGGTAGCTGGGTATTGAAGCATATTACATACGATATGCTTCAAT XhoI EcoRV | | 1321 tctcgagtcagatatcgctagccaattgataacttcgtataggatactttatacgaagtt 1380 AGAGCTCAGTCTATAGCGATCGGTTAACTATTGAAGCATATCCTATGAAATATGCTTCAA BglII | 1381 atcattgggattcttcctattttgatccaagcatcaccatcgaccctctagtccagatct 1440 TAGTAACCCTAAGAAGGATAAAACTAGGTTCGTAGTGGTAGCTGGGAGATCAGGTCTAGA loxP other(1453,1486)>>> | 1441 caccatcgacccataacttcgtatagcatacattatacgaagttatatggtgagcaaggg 1500 GTGGTAGCTGGGTATTGAAGCATATCGTATGTAATATGCTTCAATATACCACTCGTTCCC XbaI | 1501 cgattctagaaacttgtttattgcagcttataatggttacaaataaagcaatagcatcac 1560 GCTAAGATCTTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTG 1561 aaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcat 1620 TTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTA SV40 prom(1639,1907)>>> | 1621 caatgtatcttaaagcttggtgtggaaagtccccaggctccccagcaggcagaagtatgc 1680 GTTACATAGAATTTCGAACCACACCTTTCAGGGGTCCGAGGGGTCGTCCGTCTTCATACG 1681 aaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcag 1740 TTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCCGAGGGGTCGTC 1741 gcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactc 1800 CGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGGCGGGGATTGAG SV40 origin(1806,1883)>>> | 1801 cgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaa 1860 GCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGTACCGACTGATT SfiI | ORF_1 rf(1)(1873,2706)>>> | | 1861 ttttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagt 1920 AAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAAGGTCTTCATCA 1921 gaggaggcttttttggaggccgccaccAtggagagcgacgagagcggcctgcccgccatg 1980 CTCCTCCGAAAAAACCTCCGGCGGTGGTACCTCTCGCTGCTCTCGCCGGACGGGCGGTAC 1981 gagatcgagtgccgcatcaccggcaccctgaacggcgtggagttcgagctggtgggcggc 2040 CTCTAGCTCACGGCGTAGTGGCCGTGGGACTTGCCGCACCTCAAGCTCGACCACCCGCCG 2041 ggagagggcacccccaagcagggccgcatgaccaacaagatgaagagcaccaaaggcgcc 2100 CCTCTCCCGTGGGGGTTCGTCCCGGCGTACTGGTTGTTCTACTTCTCGTGGTTTCCGCGG 2101 ctgaccttcagcccctacctgctgagccacgtgatgggctacggcttctaccacttcggc 2160 GACTGGAAGTCGGGGATGGACGACTCGGTGCACTACCCGATGCCGAAGATGGTGAAGCCG 2161 acctaccccagcggctacgagaaccccttcctgcacgccatcaacaacggcggctacacc 2220 TGGATGGGGTCGCCGATGCTCTTGGGGAAGGACGTGCGGTAGTTGTTGCCGCCGATGTGG 2221 aacacccgcatcgagaagtacgaggacggcggcgtgctgcacgtgagcttcagctaccgc 2280 TTGTGGGCGTAGCTCTTCATGCTCCTGCCGCCGCACGACGTGCACTCGAAGTCGATGGCG 2281 tacgaggccggccgcgtgatcggcgacttcaaggtggtgggcaccggcttccccgaggac 2340 ATGCTCCGGCCGGCGCACTAGCCGCTGAAGTTCCACCACCCGTGGCCGAAGGGGCTCCTG 2341 agcgtgatcttcaccgacaagatcatccgcagcaacgccaccgtggagcacctgcacccc 2400 TCGCACTAGAAGTGGCTGTTCTAGTAGGCGTCGTTGCGGTGGCACCTCGTGGACGTGGGG 2401 atgggcgataacgtgctggtgggcagcttcgcccgcaccttcagcctgcgcgacggcggc 2460 TACCCGCTATTGCACGACCACCCGTCGAAGCGGGCGTGGAAGTCGGACGCGCTGCCGCCG 2461 tactacagcttcgtggtggacagccacatgcacttcaagagcgccatccaccccagcatc 2520 ATGATGTCGAAGCACCACCTGTCGGTGTACGTGAAGTTCTCGCGGTAGGTGGGGTCGTAG PstI ApaI | | 2521 ctgcagaacgggggccccatgttcgccttccgccgcgtggaggagctgcacagcaacacc 2580 GACGTCTTGCCCCCGGGGTACAAGCGGAAGGCGGCGCACCTCCTCGACGTGTCGTTGTGG 2581 gagctgggcatcgtggagtaccagcacgccttcaagacccccatcgccttcgccagatcc 2640 CTCGACCCGTAGCACCTCATGGTCGTGCGGAAGTTCTGGGGGTAGCGGAAGCGGTCTAGG 2641 cgcgctcagtcgtccaattctgccgtggacggcaccgccggacccggctccaccggatct 2700 GCGCGAGTCAGCAGGTTAAGACGGCACCTGCCGTGGCGGCCTGGGCCGAGGTGGCCTAGA 2701 cgctgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttcct 2760 GCGACTGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGA 2761 tgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgc 2820 ACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCG 2821 attgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaaggggg 2880 TAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCC 2881 aggattgggaagagaatagcaggcatgctggggaaagcttaggaacccctagtgatggag 2940 TCCTAACCCTTCTCTTATCGTCCGTACGACCCCTTTCGAATCCTTGGGGATCACTACCTC 2941 ttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcc 3000 AACCGGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCCCGCTGGTTTCCAGCGG BamHI | 3001 cgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagggatcccggt 3060 GCTGCGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCGCGCGTCCCTAGGGCCA 3061 gtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcct 3120 CACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGA 3121 cgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaa 3180 GCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTT 3181 aggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaa 3240 TCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTT pBR322 origin(3275,3894)<<< | 3241 aaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggc 3300 TTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCG 3301 tccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccga 3360 AGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCT 3361 caggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttc 3420 GTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAG 3421 cgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgcttt 3480 GCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAA 3481 ctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggct 3540 GAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGA 3541 gtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttg 3600 CACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAAC 3601 agtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggatta 3660 TCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAAT 3661 gcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct 3720 CGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGA 3721 acactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaa 3780 TGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTT 3781 gagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgttt 3840 CTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAA 3841 gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttcta 3900 CGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGAT 3901 cggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattat 3960 GCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATA 3961 caaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaa 4020 GTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTT 4021 gtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatct 4080 CATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGA 4081 cagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataacta 4140 GTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGAT 4141 cgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgct 4200 GCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGA 4201 caccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtg 4260 GTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCAC 4261 gtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaa 4320 CAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATT 4321 gtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctagatcct 4380 CATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGA 4381 tttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactg 4440 AAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGAC 4441 ggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggct 4500 CCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGA NEOKAN prom(4547,4596)>>> | 4501 tacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagc 4560 ATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCG 4561 tggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttctcgcc 4620 ACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGG BclI | 4621 gccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgtt 4680 CGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAA NTP_II marker(4688,5476)>>> | ORF_2 rf(2)(4685,5479)>>> | | 4681 tcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggct 4740 AGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGA 4741 attcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggct 4800 TAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGA 4801 gtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatga 4860 CAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACT 4861 actgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagc 4920 TGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCG 4921 tgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggg 4980 ACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCC 4981 gcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgc 5040 CGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACG 5041 aatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaaca 5100 TTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGT 5101 tcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctgga 5160 AGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCT 5161 cgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgcc 5220 GCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGG 5221 cgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtgga 5280 GCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCT 5281 aaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatca 5340 TTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGT 5341 ggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccg 5400 CCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGC 5401 cttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgcct 5460 GAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGA 5461 tcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaacc 5520 AGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGG 5521 aataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgatagggttga 5580 TTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACT f1 origin(5584,5889)<<< | 5581 gtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaag 5640 CACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTC 5641 ggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatcaagtt 5700 CCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAA 5701 ttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccgattta 5760 AAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAAT 5761 gagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggag 5820 CTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTC 5821 cgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgcgc 5880 GCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCG 5881 gcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaat 5940 CGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTA 5941 taacatcatcaataatatacctt 5963 ATTGTAGTAGTTATTATATGGAA
LOCUS dna 5963 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" misc_binding 833..838 /dbxref="REBASE:NdeI" misc_binding 1168..1173 /dbxref="REBASE:KpnI" Other Gene 1288..1321 /gene="loxP other" misc_binding 1322..1327 /dbxref="REBASE:XhoI" misc_binding 1331..1336 /dbxref="REBASE:EcoRV" misc_binding 1435..1440 /dbxref="REBASE:BglII" Other Gene 1453..1486 /gene="loxP other" misc_binding 1505..1510 /dbxref="REBASE:XbaI" Promoter 1639..1907 /gene="SV40 prom" Rep_Origin 1806..1883 /gene="SV40 origin" ORF 1873..2706 /sequence="ORF_1 rf(1)" misc_binding 1886..1898 /dbxref="REBASE:SfiI" misc_binding 2521..2526 /dbxref="REBASE:PstI" misc_binding 2532..2537 /dbxref="REBASE:ApaI" misc_binding 3051..3056 /dbxref="REBASE:BamHI" Rep_Origin 3275..3894 /gene="pBR322 origin" Promoter 4547..4596 /gene="NEOKAN prom" misc_binding 4654..4659 /dbxref="REBASE:BclI" ORF 4685..5479 /sequence="ORF_2 rf(2)" Marker 4688..5476 /gene="NTP_II marker" Rep_Origin 5584..5889 /gene="f1 origin" BASE COUNT 1428 a 1590 c 1601 g 1344 t 0 others