Scrambled siRNA GFP Lentivector
Click to enlarge image
Cat. No.
LV015-G
Unit
500ng
Price
$90.00
Cat. No.
LV7-LV015-G
Unit
4 x 500 µl
Price
$340.00
Specifications
Print Custom Datasheet
Documents
Supporting Protocol
FAQs
| What is the difference between Retro-, Lenti-, and Adeno- viruses? | |
|
Retrovirus: Classic, can integrate into the genome but with low transduction efficiency. They are useful for gene transfer and protein expression in cells that have low transfection efficiency with other transfection reagents. Lentivirus: Can integrate into the genome with relatively high transduction efficiency and they are very useful for cells that have low transfection efficiency with other transfection reagents. No special competent cells required, as they are stable plasmids. Lentiviruses are a powerful tool for stable gene transfer to both dividing and non-dividing cells in vitro and in vivo. Adenovirus: Only work transiently (about 7 days) but have almost 100% transduction efficiency. Adenoviruses can infect a broad range of cell types with the highest efficiency and infection is not dependent on active host cell division. A second key feature is that high virus titers and high-level gene expression can be obtained in most mammalian cells.
|
| What are the correct concentration units for each recombinant viral particle? | |
|
For lentiviruses and retroviruses, they are measured in CFU/ml (colony-forming units per millilitre). Transduction with lentiviruses and retroviruses can cause the formation of colonies, which can be quantified for concentration. For AAV the titer is measured as genome copies per mL (GC/mL). Adenoviruses are measured as PFU/ml (plaque-forming units per millilitre). Transduction with adenoviruses will kill packaging cells, forming plaques in the process for quantification. The concentration for all three types of viruses can also be classified as IU/ml (Infectious Units/ml). Ultimately, the units refers to the viral particles and different units reflect the different assays involved.
|
| What do I use to check if my cells were successfully immortalized by the SV40 agent? | |
|
We have an SV40 T antibody that can be used for the western blot analysis. The catalog number is G202.
Otherwise, a qPCR primer can be designed on the SV40 gene for qPCR analysis. The sequence can be found in the link below:
http://www.abmgood.com/pLenti%20SV40-Vector-Location-Map.html
|
| What are the primers to use for SV40 identification? | |
|
SV40 Forward Primer Sequence
5’ ACTGAGGGGCCTGAAATGA
SV40 Reverse Primer Sequence
5’ GACTCAGGGCATGAAACAGG
These are qPCR primers and the band size is 61 bp.
|
| What advantages / disadvantages exist between the Lenti-SV40, -SV40T, and SV40T+t vectors? | |
|
There are simply differences in the content of all vectors due to customer demand for variety. Lenti-SV40 will contain the whole SV40 gene, -SV40T, the large T Antigen only, and -SV40T&t the large and small T antigens only.
It is up to the end user to decide which vectors will best suit their project, however we have successfully used Lenti-SV40 (whole gene) in a wide range of immortalization projects.
|
| What is the accession number for the SV40? | |
|
The SV40 covers the entire genome and the accession number is J02400.1. You can use this information to design primers for conventional PCR as well.
|
| How long after transduction can the infection efficiency be observed? | |
|
You can observe transduction efficiency from 48 hours up to 5 days after infection.
|
| What are the primers to use for SV40T and SV40T tsA58 detection? | |
|
PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.
|
| What is the sequence of the SV40 large T antigen? | |
|
This information can be accessed on this page by clicking on "pLenti-SV40-T" under vector map. The Large T antigen is at position 5079-5927.
|
| For G221 and LV620, what does the 'V12' in RasV12 mean? | |
|
The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).
|
| Where is the SV40T tsA58 gene sequence? | |
|
The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.
|
References
- Jin, Q;Liu, G;Domeier, PP;Ding, W;Mulder, KM;, et al. "Decreased tumor progression and invasion by a novel anti-cell motility target for human colorectal carcinoma cells" PLoS ONE 8-6:e66439 (2013). DOI: 10.1371. PubMed: 23755307.
- Liang, Q;Dexheimer, TS;Zhang, P;Rosenthal, AS;Villamil, MA;You, C;Zhang, Q;Chen, J;Ott, CA;Sun, H;Luci, DK;Yuan, B;Simeonov, A;Jadhav, A;Xiao, H;Wang, Y;Maloney, DJ;Zhuang, Z;, et al. "A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses" Nat. Chem. Biol. 10-4:298-304 (2014). PubMed: 24531842.
- Bayazitov, IT et al. "Forward suppression in the auditory cortex is caused by the Ca(v)3.1 calcium channel-mediated switch from bursting to tonic firing at thalamocortical projections" J. Neurosci. 33:18940 - 50 (2013). DOI: 10.1523/JNEUROSCI.3335-13.2013. PubMed: 24285899. Application: siRNA.
- Yang, Z et al. "Screening with a Novel Cell-Based Assay for TAZ Activators Identifies a Compound That Enhances Myogenesis in C2C12 Cells and Facilitates Muscle Repair in a Muscle Injury Model" Mol Cell Biol. 34 (9):1607-1621 (2014). DOI: 10.1128/MCB.01346-13. PubMed: 24550007. Application: siRNA Control.
- Lellahi, SM. "POU3f2 in human gliomas - Expression pattern and functional role" Bergen Open Research Archive Thesis: (2014).
- Wang, B et al. "BET Bromodomain Blockade Mitigates Intimal Hyperplasia in Rat Carotid Arteries" J. Ebiom 2(11):1650-1661 (2015). DOI: 10.1016/j.ebiom.2015.09.045.
- Liu, XB et al. "Pituitary tumor transforming gene PTTG2 induces psoriasis by regulating vimentin and E-cadherin expression" Int. J. Clin. Exp. Path. 8(9):10887-10893 (2015). PubMed: 26617803.
- Yang, Z et al. "MicroRNA expression profiles in human adipose-derived stem cells during chondrogenic differentiation" Int. J. Mol. Med. 3:579-586 (2015). DOI: 10.3892/ijmm.2014.2051. PubMed: PMC4314422.
- Chen, Y et al. "IL-6 signaling promotes DNA repair and prevents apoptosis in CD133+ stem-like cells of lung cancer after radiation" Radiat Oncol 227: (2015). DOI: 10.1186/s13014-015-0534-1. PubMed: PMC4647293.
- Shakya, A et al. "Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion." Mol Cell Biol 6:1014-25 (2015). DOI: 10.1128/MCB.01105-14. PubMed: 25582194 .
- Qu, J et al. "Kindlin-3 interacts with the ribosome and regulates c-Myc expression required for proliferation of chronic myeloid leukemia cells" Scientific Reports 5: (2015). DOI: 10.1038/srep18491. Application: siRNA.
- Shimizu, T et al. "Microglia-Induced Activation of Noncanonical Wnt Signaling Aggravates Neurodegeneration in Demyelinating Disorders" Mol. Cell. Biol. 36.21:2728-2741 (2016). DOI: 10.1128/MCB.00139-16. Application: Research.
- Devaraju, P et al. "Haploinsufficiency of the 22q11.2 microdeletion gene Mrpl40 disrupts short-term synaptic plasticity and working memory through dysregulation of mitochondrial calcium" Mol. Psychiatry : (2016). DOI: 10.1038/mp.2016.75. Application: siRNA.
- Rong, H et al. ""Notch signaling suppresses regulatory T cell function in murine experimental autoimmune uveitis "" Immunology 149.4:447-459 (2016). DOI: 10.1111/imm.12663.
- Pandya, et al. "Transglutaminase 2 overexpression induces depressive-like behavior and impaired TrkB signaling in mice" Mol. Psychiatry 2016: (2016). DOI: 10.1038/mp.2016.145. Application: Lentivirus Production.
- Zhang, et al. "The construction and proliferative effects of a lentiviral vector capable of stably overexpressing SPINK1 gene in human pancreatic cancer AsPC-1 cell line" Tumour Biology 37.5:5847 (2016). DOI: 10.1007/s13277-015-4405-z. Application: Generation of lentiviral vectors.
Other Products
E. coli
GFP
Human
Human (H. sapiens)
Luc
Luciola italica
Mouse
Other
Photinus pyralis
Renilla reniformis
Serotype 1
Serotype 10
Serotype 2
Serotype 3
Serotype 4
Serotype 5
Serotype 6
Serotype 7
Serotype 8
Serotype 9
Lentiviral
Vector
AAV
Vector
Adenovirus
Vector
Protein
Vector
ORF
Vector
siRNA
Vector
miRNA
Vector
3'UTR
Vector
CRISPR Knockout
Vector
CRISPR Activation
Vector
Control Vectors & Viruses
Vector
circRNA
Vector
Internal
Vector