Recombinant Rat IL1B (E. coli)
Cat. No.
Z300075
Unit
Price
$89.00
Specifications
| Description | Monocytes are the main source of secreted IL-1. They express predominantly IL-1-beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T cells is expressed predominantly on T cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B cells, granulocytes, and macrophages. It is expressed predominantly on B cells and cells of the myelomonocytic lineage and is encoded by a separate gene. |
| SKU | Z300075 |
| Unit quantity | 10 µg |
| Family | IL-1 Family |
| Aliases |
IL-1b Recombinant Rat Interleukin-1 Beta (IL1B) |
| Gene Symbol | IL1B |
| Gene ID | 24494 |
| Accession Number | Q63264 |
| Source | E. coli |
| Species | Rat (R. norvegicus) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 17 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Rat IL-1 beta was lyophilized from a 0.2 μm filtered solution in PBS, pH 7. |
| Function | The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by murine D10S cells is ≤ 2.0 pg/mL, corresponding to a specific activity of ≥ 5.0 x 10^8 units/mg. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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