Recombinant Mouse THPO
Cat. No.
Z200535
Unit
Price
$246.00
Specifications
| Description | Thrombopoietin, known also as TSF (thrombopoiesis stimulating factor), for mediator substances that function as natural stimulators of megakaryocytopoiesis and thus, promote the differentiation of platelets. Thrombopoietin stimulates an increase in the sizes and numbers of megakaryocytes. It also increases the number of small acetyl-cholinesterase positive cells that are early precursor cells of the megakaryocytic lineage. Thrombopoietin is a protein of 332 amino acids that shows significant homology with Epo (erythropoietin) in its N-terminal domain. A comparison of human and porcine Thrombopoietin sequences shows 83% identity between the erythropoietin-like domains but only 67% between the C-terminal domains. |
| SKU | Z200535 |
| Unit quantity | 10 µg |
| Family | HDGF/S-100/EPO(TPO) Families |
| Aliases |
C-mpl ligand, ML, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, MGDF, Myeloproliferative leukemia virus oncogene ligand Recombinant Mouse Thrombopoietin (THPO) |
| Gene Symbol | THPO |
| Gene ID | 21832 |
| Accession Number | P40226 |
| Source | E. coli |
| Species | Mouse (M. musculus) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 19 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Recombinant mouse TPO was lyophilized from a 0.2 μm filtered PBS solution. |
| Function | The ED(50) was determined by the dose-dependent proliferation of human MO7e cells was found to be less than 1.0 ng/mL. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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