Recombinant Mouse IL10 (E. coli)
Cat. No.
Z200115
Unit
Price
$110.00
Specifications
| Description | IL10 is produced by mouse Th2 cells following their stimulation by lectins. The main source for B cell derived IL10 in mice are Ly1 B cells that express CD5 (Ly1) and CD11. In humans IL10 is produced by activated CD8(+) peripheral blood T cells, by T helper CD4(+) T cell clones after both antigen-specific and polyclonal activation, by B cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL10 is a homodimeric protein with subunits having a length of 160 amino acids. Human IL10 shows 73% amino acid homology with mouse IL10. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL-2 and TNF-beta in Th1 T helper subpopulations of T cells but not of Th2 helper cells. This activity is antagonized by IL-4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL-12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of, Th1 and Th2 cells. |
| SKU | Z200115 |
| Unit quantity | 10 µg |
| Family | IL-10/Interferon Family |
| Aliases |
IL-10, Cytokine synthesis inhibitory factor, CSIF Recombinant Mouse Interleukin-10 (IL10) |
| Gene Symbol | IL10 |
| Gene ID | 16153 |
| Accession Number | P18893 |
| Source | E. coli |
| Species | Mouse (M. musculus) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 19 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.0) |
| Function | The ED50 was determined by the dose-dependent (with IL-4) proliferation of murine MC/9 cells and was found to be < 2.0 ng/mL |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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