Recombinant Mouse GM-CSF (CSF2) (E. coli)
Cat. No.
Z200075
Unit
Price
$199.00
Specifications
| Description | GM-CSF is a monomeric cytokine with two glycosylation sites that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. CSF2 is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. The signalling ‘GM-CSF:receptor’ complex is a dodecamer of two hexamers of two alpha, two beta, and two ligand subunits. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo. Recombinant Mouse CSF2 is a non-glycosylated, monomeric protein. |
| SKU | Z200075 |
| Unit quantity | 20 µg |
| Family | Other Growth Factors/Cytokines |
| Aliases |
GMCSF, Granulocyte-macrophage colony-stimulating factor, CSF2 Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor (CSF2) |
| Gene Symbol | CSF2 |
| Gene ID | 12981 |
| Accession Number | P01587 |
| Source | E. coli |
| Species | Mouse (M. musculus) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 14 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from 0.2 μm filtered solution in Tris, pH 7.5 and NaCl |
| Function | The ED50 as determined by the dose-dependent stimulation BaF3 cells was < 0.1 ng/mL |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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