Recombinant Human TGFA
Specifications
Description | An EGF family member, TGF-A is a cytokine that is produced following the protease digestion of its trans-membrane precursor proTGF-A. TGF-A is expressed both in normal tissues during embryogenesis and in adult tissues, such as the brain, keratinocytes, and macrophages and at high levels in transformed and tumour cells. |
SKU | Z101935 |
Unit quantity | 20 µg |
Family | EGF Family |
Aliases |
TGF-alpha, TGF-A, isoform CRA_d Recombinant Human Transforming Growth Factor Alpha (TGFA) |
Gene Symbol | TGFA |
Gene ID | 7039 |
Accession Number | P01135 |
Source | E. coli |
Species | Human (H. sapiens) |
Appearance | Lyophilized Powder |
Molecular Weight | 5.8 kDa |
Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
Purity | >95% as determined by SDS-PAGE |
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA |
Function | Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED(50) for this effect is typically 0.10.4 ng/mL. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | For research use only. Not for diagnostic or therapeutic use. |
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