Recombinant Human Nodal
Specifications
Description | Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development. |
SKU | Z102485 |
Unit quantity | 25 µg |
Family | TGF-beta Super-family |
Aliases | Nodal homolog |
Gene Symbol | NODAL |
Gene ID | 4838 |
Accession Number | Q96S42 |
Source | E. coli |
Species | Human (H. sapiens) |
Appearance | Lyophilized Powder |
Molecular Weight | 13.1 kDa |
Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
Purity | >95% as determined by SDS-PAGE |
Formulation | Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein) |
Function | 100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells |
Reconstitution | Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin) |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | For research use only. Not for diagnostic or therapeutic use. |
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