Recombinant Human Midkine (NEGF2) (E. coli)
Cat. No.
Z102175
Unit
Price
$100.00
Specifications
| Description | Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines. |
| SKU | Z102175 |
| Unit quantity | 20 µg |
| Family | VEGF/PGDF/Pleiotrophin Family |
| Aliases | MK, NEGF-2 |
| Gene Symbol | MDK |
| Gene ID | 4192 |
| Accession Number | P21741 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 14 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Function | The ED(50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL. |
| Reconstitution | Reconstitute at 0.1 mg/ml in sterile PBS |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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