Recombinant Human HB-EGF (Heparin Binding EGF) (E. coli)
Cat. No.
Z102155
Unit
Price
$89.00
Specifications
| Description | Human HBEGF (Heparin Binding EGF like growth factor) is a member of the EGF family of peptide growth factors that signal through the EGF receptor, and stimulates the proliferation of smooth muscle cells, fibroblasts, epithelial cells, and keratinocytes. HBEGF is expressed in many cell types and tissues, including bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, glomerular podocytes, keratinocytes, macrophages and certain tumor cells. Mature HBEGF is a soluble peptide that arises from the proteolytic processing of the 208 amino acid type-I transmembrane form and exhibits the ability to specifically bind heparin and heparin sulfate proteoglycans. |
| SKU | Z102155 |
| Unit quantity | 50 µg |
| Family | EGF Family |
| Aliases | Heparin Binding EGF-like growth factor, HBEGF, Diphtheria toxin receptor, DTR |
| Gene Symbol | HBEGF |
| Gene ID | 1839 |
| Accession Number | Q99075 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 10.1 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Function | The ED50, as determined by a cell proliferation assay (using balb/c 3T3 cells) is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg. |
| Reconstitution | It is recommended to reconstitute HBEGF in sterile PBS at 250 μg/mL. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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