Recombinant Human GDF3 (E. coli)
Cat. No.
Z101735
Unit
Price
$205.00
Specifications
| Description | GDF-3 is a growth and differentiation factor which belongs to the TGF-beta superfamily. GDF-3 and GDF-9 are the only two members of the family which do not form disulfide-linked dimers. GDF-3 has been found in adult bone marrow, spleen, thymus, and adipose tissue. Although signaling in all other members of the family involve the BMP receptors ALK1,2,3, and 6, GDF-3 signals through BMP receptors ALK4 and 7. |
| SKU | Z101735 |
| Unit quantity | 20 µg |
| Family | TGF-beta Super-family |
| Aliases |
GDF-3 Recombinant Human Growth Differentiation Factor 3 (GDF3) |
| Gene Symbol | GDF3 |
| Gene ID | 9573 |
| Accession Number | Q9NR23 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 13 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from solution in 30mM Acetate buffer, pH 4 |
| Function | Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. |
| Reconstitution | A quick spin of the vial followed by reconstitution in sterile 100mM Acetate buffer, pH 4 at a concentration of 0.5 mg/mL. Please note that in higher concentrations the solubility of GDF3 is limited. The protein is not lypholized in a filtered solution. Please sterile filter before using it in the cell culture. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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