Recombinant Human FGF8 (E. coli)
Specifications
Description | FGF-8 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-8 can be spliced in four different ways. The products of the FGF-8 splicing events are as follows: FGF8A, 8B, 8E and 8F. This recombinant product represents isoform 8B. |
SKU | Z101705 |
Unit quantity | 50 µg |
Family | FGF Family |
Aliases |
FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8 Recombinant Human Fibroblast Growth Factor 8 (FGF8) |
Gene Symbol | FGF8 |
Gene ID | 2253 |
Accession Number | P55075 |
Source | E. coli |
Species | Human (H. sapiens) |
Appearance | Lyophilized Powder |
Molecular Weight | 22.5 kDa |
Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
Purity | >95% as determined by SDS-PAGE |
Formulation | Lyophilized from a 0.2 μm filtered solution in 20mM phosphate buffer, 0.2 mM EDTA and 1M NaCl (pH 8.0) |
Function | The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml. |
Reconstitution | A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | For research use only. Not for diagnostic or therapeutic use. |
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