Recombinant Human DHH (E. coli)
Cat. No.
Z101695
Unit
Price
$89.00
Specifications
| Description | The Desert Hedgehog protein is one of the three Hedgehog family proteins that is highly conserved and plays a role in many developmental processes in animals. DHH become biologically active following its autocatalytic cleavage. There is a high degree of residue homology between Sonic Hedgehog (SHH), Indian Hedgehog (IHH), and DHH. Despite their individual levels of expression and unique biological roles, the Hedgehog family members all use the same signalling pathway, and therefore, may be used interchangeably for some experimental purposes. |
| SKU | Z101695 |
| Unit quantity | 50 µg |
| Family | Hedgehog Family |
| Aliases |
Desert Hedgehog Protein, DHH Recombinant Human Desert Hedgehog (DHH) |
| Gene Symbol | DHH |
| Gene ID | 50846 |
| Accession Number | O43323 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 20 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 0.2 mM DTT |
| Function | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 15-45 µg/mL |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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