Recombinant Human CCL21 (E. coli)
Cat. No.
Z100195
Unit
Price
$89.00
Specifications
| Description | CCL21, which is a member of the family of CC-Chemokines, has a unique 36 (murine) or 37 (human) amino acid carboxyl-terminal extension not seen in other chemokines. CCL21 selectively stimulates the chemotaxis of adult T lymphocytes and is expressed preferentially in lymph node tissue. CCL21 is critical for adult T cell adhesion to high endothelial venules in lymph nodes, a rate-limiting step for T cell trafficking through nodal tissue. |
| SKU | Z100195 |
| Unit quantity | 20 µg |
| Family | Chemokine-beta Family (CC) |
| Aliases |
C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, SLC, Small-inducible cytokine A21, SCYA21 Recombinant Human Secondary Lymphoid-Tissue Chemokine (CCL21) |
| Gene Symbol | CCL21 |
| Gene ID | 6366 |
| Accession Number | O00585 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 12 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Recombinant CCL21 was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5. |
| Function | Determined by its ability to chemoattract total lymphocyte population using a concentration range of 10-100 ng/mL. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
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