Ni-IDA Agarose Resins
Specifications
Cat. No. | G250 |
Name | Ni-IDA Agarose Resins |
Price | $73.00 |
Unit | 5.0 ml |
Category | Buffers & Chemicals |
Description |
Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni2+ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.
Special Features
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Application |
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Storage Condition |
Protein G Agarose Resin should be stored at 2-8°C. Do not freeze. |
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