Custom 5’ UTR Reporter Vectors

abm’s 5’ UTR Reporter Vectors consist of the 5’ UTR (Untranslated Region) of the gene of your choice preceding a GFP or Luciferase reporter, driven by the β-actin promoter. These vectors are ideal for studying the regulation of protein expression by 5’ UTR elements. While 3’ UTRs are the major focus of regulatory research, 5’ UTRs have also been found to mediate translational regulation via elements such as upstream start codons (uAUGs), upstream open reading frames (uORFs), secondary structures, RNA binding protein (RBP) motifs, and miRNA binding.

Simply inquire with us below to get started on your Custom 5' UTR Vector.

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Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL

Service Details

PRODUCT	REPORTER	AMOUNT	Cat. No.	PRICE
Custom 5' UTR Reporter Lentiviral Vector Subcloning (GFP)	EGFP	1.0 µg	C451	$350.00
Custom 5' UTR Reporter Lentiviral Vector Subcloning (Luc)	Firefly Luciferase	1.0 µg	C452	$350.00

CONTROLS	REPORTER	AMOUNT	Cat. No.	PRICE
Blank 5' UTR Reporter Lentiviral Vector (GFP)	EGFP	1.0 µg	LV657	$150.00
Blank 5' UTR Reporter Lentiviral Vector (Luc)	Firefly Luciferase	1.0 µg	LV658	$150.00

ADD-ONS	AMOUNT	Cat. No.	PRICE
Site Directed Mutagenesis Service	Single Clone	C047	$150.00
Plasmid Amplification (10-20 µg)	10‑20 µg	C309	$75.00
Plasmid Amplification (80-100 µg)	80‑100 µg	C310	$150.00
Bacterial Agar Stab	1 Clone	C315	$40.00
Custom Lentivirus Packaging Service	10⁷‑10¹⁰ IU/ml	Click here	From $395.00

Additional Info


pLenti-5'UTR-GFP Vector	pLenti-5'UTR-Luc Vector

All of abm’s UTR reporter vectors are available as ready-to-use plasmids, or they can be packaged into lentivirus. Our UTR reporter vectors can be directly transfected into target cells for transient expression, while lentivirus allows for difficult-to-transfect cells, such as primary cells, to be efficiently transduced for stable, long-term expression.

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Citations

01	Xu, Y et al. "Bile acid and cigarette smoke enhance the aggressive phenotype of esophageal adenocarcinoma cells by downregulation of the mitochondrial uncoupling protein-2." Oncotarget 8.60:101057–101071. (2017). DOI: 10.18632/oncotarget.22380 Application: Lentiviral transfection.